Journal
BIOSENSORS & BIOELECTRONICS
Volume 228, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2023.115200
Keywords
Interfacial deposition; Silver nanoparticle; Polyphenol; Nanozyme; SERS; Colloidal sphere
Ask authors/readers for more resources
In this study, a structurally tunable nanomaterials platform for indirect detection of glutathione (GSH) through surface enhanced Raman scattering (SERS) was developed. The CuTA@Ag nanostructures demonstrated satisfactory SERS performance with a low detection limit of 10-7 M for GSH, and were able to accurately and reliably detect the glutathione content in cancer cells.
The low polarization and low Raman cross section characteristics of glutathione (GSH) make it challenging to directly detect GSH molecules through surface enhanced Raman scattering (SERS) technology. Development of nanostructures for indirect detection of GSH applied to the SERS platform is of great interest. Herein, silver nanoparticles (Ag NPs)/copper-polyphenol colloidal spheres (denoted as CuTA@Ag) with adjustable Ag NPs coverage are prepared by deposition of Ag NPs on the metal-polyphenol colloidal spheres via an interfacial polyphenol reduction method. The size and density of the Ag NPs deposited on the out layer can be readily adjusted by tailoring the concentrations of silver precursor. It leads to activity difference for the nanozyme and SERS characteristics. The SERS properties of the obtained CuTA@Ag are studied using oxTMB, catalytic products of nanozyme, as the probing molecules. They provide satisfactory SERS performance with a low detection limit of 10- 7 M (S/N = 3) and linear determination in the 1-100 & mu;M range for GSH. Moreover, it is further able to detect the glutathione content in cancer cells with well accurate and reproducible capability, catching the signs of rising cancer marker levels. This work proposes structurally tunable nanomaterials platform for a catalytic-based SERS assay, which is expected to utilize the high sensitivity of SERS tool for GSH detection in the cellular environment.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available