4.8 Article

Super-resolution dynamic tracking of cellular lipid droplets employing with a photostable deep red fluorogenic probe

Journal

BIOSENSORS & BIOELECTRONICS
Volume 229, Issue -, Pages -

Publisher

ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2023.115243

Keywords

Fluorescence imaging; Live-cell imaging; Super-resolution imaging; Fluorescent probes; Lipid droplets

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A new fluorescent probe called Lipi-Deep Red has been developed for super-resolution imaging of lipid droplets (LDs), allowing for visualization and study of LDs dynamics and interaction with mitochondria at the nanoscale level. This probe offers strong deep red/NIR emission, high LDs specificity, and excellent photostability, enabling precise monitoring and visualization of LD fusion processes and the discovery of two contact models between LDs and mitochondria.
Lipid droplets (LDs) are critical organelles involved in many physiological processes in eukaryotic cells. To visualize and study LDs, particular the small/nascent LDs, the emerging super-resolution fluorescence imaging techniques with nanoscale resolution would be much more powerful in comparison to the conventional confocal/ wide-field imaging techniques. However, directly limited by the availability of advanced LDs probes, super -resolution fluorescence imaging of LDs is a practically challenging task. In this context, a superior LDs fluores-cent probe named Lipi-Deep Red is newly developed for structured illumination microscopy (SIM) super -resolution imaging. This fluorescent probe features with the advantages of strong deep red/NIR emission, flu-orogenic character, high LDs specificity, and outstanding photostability. These advantages enable the fluorescent probe to be finely applied in SIM super-resolution imaging, e.g. time-lapse imaging (up to 1000 frames) to monitor the LDs dynamics at nanoscale (159 nm), two-color time-lapse imaging to discover the nearby contact/ interaction between LDs and mitochondria. Consequently, the fusion processes of LDs are impressively visualized at a high spatial and temporal resolution. Two kinds of contact models between LDs and mitochondria (dynamic contact and stable contact) newly proposed in the recent literatures are successfully revealed.

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