4.8 Article

A universal and specific RNA biosensor via DNA circuit-mediated PAM-independent CRISPR/Cas12a and PolyA-rolling circle amplification

Related references

Note: Only part of the references are listed.
Article Chemistry, Analytical

Bis-enzyme cascade CRISPR-Cas12a platform for miRNA detection

Zixuan Guo et al.

Summary: MicroRNA (miRNA) plays a crucial role in the development of various diseases, making it important to have a reliable detection method. In this study, a Bis-enzyme cascade Platform (BPTC) combining T7 RNA polymerase and CRISPR-Cas12a was proposed for miRNA detection. The BPTC showed high sensitivity and excellent specificity, providing a new strategy for the design of CRISPR-Cas-based miRNA biosensing platforms.

TALANTA (2023)

Article Biochemistry & Molecular Biology

Photocaged amplified FRET nanoflares: spatiotemporal controllable of mRNA-powered nanomachines for precise and sensitive microRNA imaging in live cells

Jing Li et al.

Summary: This article introduces a novel photocaged amplified FRET nanoflare (PAFN) for precise and sensitive visualization and analysis of intracellular miRNA in live cells. The PAFN can be remotely activated by light and triggered by specific low-abundance miRNA, while fueled by high-abundance mRNA. It offers high spatiotemporal control and ratiometric signal amplification, enabling precise and sensitive detection of specific miRNA in live cells. This strategy may have significant implications for biological and medical imaging.

NUCLEIC ACIDS RESEARCH (2022)

Review Biotechnology & Applied Microbiology

CRISPR-Cas-mediated diagnostics

Luoxi Li et al.

Summary: This article discusses the application of CRISPR-Cas technology in the field of molecular diagnostics and emphasizes the revolutionary advancements it brings.

TRENDS IN BIOTECHNOLOGY (2022)

Article Chemistry, Analytical

Enzyme Kinetics and Detector Sensitivity Determine Limits of Detection of Amplification-Free CRISPR-Cas12 and CRISPR-Cas13 Diagnostics

Diego A. Huyke et al.

Summary: This study quantifies the kinetic parameters and detection limits of enzymes in CRISPR-Cas12 and CRISPR-Cas13 detection, and validates the self-consistency of the measurements. The results indicate that successful target detection requires the cleavage of at least 0.1% of the fluorescent reporter molecules, which is independent of the detection method and detector sensitivity.

ANALYTICAL CHEMISTRY (2022)

Article Biophysics

PCDetection: PolyA-CRISPR/Cas12a-based miRNA detection without PAM restriction

Mingtian Zhong et al.

Summary: This article proposes a method that integrates PolyA-tailing and CRISPR/Cas12a to amplify and detect all miRNAs with high specificity and sensitivity. The practical application ability of this method was validated by miRNA analyses in multiple cancer cell samples.

BIOSENSORS & BIOELECTRONICS (2022)

Correction Cell Biology

Emerging roles and functional mechanisms of PIWI- interacting RNAs (Sept, 10.1038/s41580-022-00528-0, 2022)

Xin Wang et al.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2022)

Review Chemistry, Multidisciplinary

Functional nucleic acid biosensors utilizing rolling circle amplification

Roger M. Bialy et al.

Summary: This article discusses the design considerations for using rolling circle amplification (RCA) combined with functional nucleic acids (FNAs) to achieve ultrasensitive detection of non-nucleic acid targets. It also describes the recent progress in the development of portable optical and electrochemical point-of-care devices incorporating RCA, and summarizes the key challenges and opportunities in the field.

CHEMICAL SOCIETY REVIEWS (2022)

Review Biochemistry & Molecular Biology

Fluorescent-based nanosensors for selective detection of a wide range of biological macromolecules: A comprehensive review

Saman Sargazi et al.

Summary: Fluorescent-based bioprobes have unique attributes that make them suitable for creating highly sensitive nano-biosensors for detecting various biological and chemical agents. These sensors are superior to other analytical instrumentation techniques in terms of being biodegradable, eco-friendly, and more economical and cost-effective. This paper provides a comprehensive overview of the electrochemical sensors that use nanomaterials to detect biological macromolecules based on fluorescent emission spectra.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022)

Review Chemistry, Analytical

Rolling Circle Amplification as an Efficient Analytical Tool for Rapid Detection of Contaminants in Aqueous Environments

Kuankuan Zhang et al.

Summary: This paper introduces the principles of rolling circle amplification (RCA) technology and its application in environmental analysis. It discusses the advantages and disadvantages of this method in contaminant monitoring, as well as its potential for detecting heavy metals, organic small molecules, nucleic acids, proteins, and other targets in aqueous environments.

BIOSENSORS-BASEL (2021)

Article Chemistry, Analytical

Target-Cell-Specific Bioorthogonal and Endogenous ATP Control of Signal Amplification for Intracellular MicroRNA Imaging

Xiangdan Meng et al.

Summary: A novel DNA nano-system has been developed for signal amplification detection of miRNA within cancer cells through endogenous ATP activation of the hybridization chain reaction.

ANALYTICAL CHEMISTRY (2021)

Review Medical Laboratory Technology

rRT-PCR for SARS-CoV-2: Analytical considerations

Rezgar Rahbari et al.

Summary: The COVID-19 pandemic is a significant global health problem that requires accurate and early detection for control and treatment; real-time reverse-transcriptase polymerase-chain-reaction (rRT-PCR) is the gold standard, but false negative results and errors are possible; sources of error include pre-analytical, analytical, and post-analytical phases.

CLINICA CHIMICA ACTA (2021)

Review Chemistry, Analytical

Recent advances in rolling circle amplification-based biosensing strategies-A review

Lulu Xu et al.

Summary: RCA is an efficient enzymatic isothermal reaction using circular probes to amplify circular templates rapidly, with high specificity and the ability to generate customized functional nucleic acids for in situ detection.

ANALYTICA CHIMICA ACTA (2021)

Article Chemistry, Analytical

Universal and Programmable Rolling Circle Amplification-CRISPR/Cas12a-Mediated Immobilization-Free Electrochemical Biosensor

Min Qing et al.

Summary: The study introduced the CRISPR/Cas system into an electrochemical biosensing platform for sensitive and specific detection of disease-related molecules. By utilizing modular rolling circle amplification and CRISPR/Cas12a, signal amplification was achieved. The flexibility and programmability of this strategy allow for the detection of various targets.

ANALYTICAL CHEMISTRY (2021)

Review Cell Biology

miRNA interplay: mechanisms and consequences in cancer

Meredith Hill et al.

Summary: miRNAs can control mRNA expression and target non-coding RNAs, including long non-coding RNAs and other miRNAs. MiRNA:miRNA interaction is a form of self-regulation, with direct, indirect, and global interactions being the main modes. Understanding the self-regulation of miRNAs can help identify novel regulatory mechanisms associated with disease pathways.

DISEASE MODELS & MECHANISMS (2021)

Article Chemistry, Analytical

A Deoxyribozyme-Initiated Self-Catalytic DNA Machine for Amplified Live-Cell Imaging of MicroRNA

Yeqing Wan et al.

Summary: A self-catalytic DNA assembly (SDA) machine was developed for programmable assembly of functional DNA nanostructures in living cells, enabling sensitive detection of cancer biomarkers such as miR-21. The system combines catalytic self-assembly (CSA) and DNAzyme amplification modules to achieve a self-catalytic amplification reaction, leading to precise intracellular imaging and potential for early cancer diagnosis.

ANALYTICAL CHEMISTRY (2021)

Article Biophysics

CRISPR/cas systems redefine nucleic acid detection: Principles and methods

Meng Wang et al.

BIOSENSORS & BIOELECTRONICS (2020)

Article Multidisciplinary Sciences

Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection

Long T. Nguyen et al.

NATURE COMMUNICATIONS (2020)

Review Chemistry, Multidisciplinary

The Recent Development of Hybridization Chain Reaction Strategies in Biosensors

Chuyan Zhang et al.

ACS SENSORS (2020)

Review Biochemistry & Molecular Biology

The emerging role of the piRNA/piwi complex in cancer

Yongmei Liu et al.

MOLECULAR CANCER (2019)

Article Biophysics

Nanobiosensors: Point-of-care approaches for cancer diagnostics

Ruchita Shandilya et al.

BIOSENSORS & BIOELECTRONICS (2019)

Review Genetics & Heredity

PIWI-interacting RNAs: small RNAs with big functions

Deniz M. Ozata et al.

NATURE REVIEWS GENETICS (2019)

Article Nanoscience & Nanotechnology

On-Particle Rolling Circle Amplification-Based Core-Satellite Magnetic Superstructures for MicroRNA Detection

Bo Tian et al.

ACS APPLIED MATERIALS & INTERFACES (2018)

Article Multidisciplinary Sciences

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

Janice S. Chen et al.

SCIENCE (2018)

Review Oncology

Applications of CRISPR-Cas Enzymes in Cancer Therapeutics and Detection

Chun-Hao Huang et al.

TRENDS IN CANCER (2018)

Article Multidisciplinary Sciences

Nucleic acid detection with CRISPR-Cas13a/C2c2

Jonathan S. Gootenberg et al.

SCIENCE (2017)

Review Chemistry, Multidisciplinary

The Discovery of Rolling Circle Amplification and Rolling Circle Transcription

Michael G. Mohsen et al.

ACCOUNTS OF CHEMICAL RESEARCH (2016)

Review Genetics & Heredity

The widespread regulation of microRNA biogenesis, function and decay

Jacek Krol et al.

NATURE REVIEWS GENETICS (2010)

Article Biochemical Research Methods

Direct observation of individual endogenous protein complexes in situ by proximity ligation

Ola Soderberg et al.

NATURE METHODS (2006)