Improved enzymatic saccharification of bulrush via an efficient combination pretreatment

Glycerol (Gly) was selected as hydrogen-bond-donor for preparing ChCl-based DES (ChCl:Gly), and the mixture of ChCl:Gly (20 wt%) and NaOH (4 wt%) was utilized for combination pretreatment of bulrush at 100 degrees C for 60 min (severity factor LogRo =1.78). The effects of DES pretreatment on the chemical composition, microstructure, crystal structure, and cellulase hydrolysis were explored. NaOH-ChCl:Gly could remove lignin (80.1%) and xylan (66.8%), and the enzymatic digestibility of cellulose reached 87.9%. The accessibility of bulrush was apparently increased to 645.2 mg/g after NaOH-ChCl:Gly pretreatment. The hydrophobicity and lignin surface area were reduced to 1.56 L/g and 417 m2/g, respectively. The crystallinity of cellulose was increased from 20.8% to 55.6%, and great changes in surface morphology were observed, which explained the improvement of enzymatic hydrolysis efficiency. Overall, DES combined with alkali treatment could effectively promote the removal of lignin and xylan in bulrush, thus the relative saccharification activity was greatly affected.

Automated summary

Glycerol was used as a hydrogen-bond-donor to prepare a ChCl-based deep eutectic solvent (DES) for the combination pretreatment of bulrush. The DES pretreatment effectively removed lignin and xylan from bulrush, resulting in increased enzymatic digestibility of cellulose. The DES combined with alkali treatment significantly improved the accessibility and crystallinity of cellulose, leading to enhanced enzymatic hydrolysis efficiency.