Intrinsically disordered region of talin?s FERM domain functions as an initial PIP2 recognition site

Focal adhesions (FAs) mediate the interaction of the cytoskeleton with the extracellular matrix in a highly dynamic fashion. Talin is a central regulator, adaptor protein, and mechano-sensor of FA complexes. For recruitment and firm attachment at FAs, talin's N-terminal FERM domain binds to phosphatidylinositol 4,5-bisphosphate (PIP2)-enriched membranes. A newly published autoinhibitory structure of talin-1, where the known PIP2 interaction sites are covered up, lead us to hypothesize that a hitherto less examined loop insertion of the FERM domain acts as an additional and initial site of contact. We evaluated direct interactions of talin-1 with a PIP2 membrane by means of atomistic molecular dynamics simulations. We show that this unstructured, 33-residue-long loop strongly interacts with PIP2 and can facilitate further membrane contacts, including the ca-nonical PIP2 interactions, by serving as a flexible membrane anchor. Under force as present at FAs, the extensible FERM loop ensures talin maintains membrane contacts when pulled away from the membrane by up to 7 nm. We identify key basic residues of the anchor mediating the highly dynamic talin-membrane interaction. Our results put forward an intrinsically disordered loop as a key and highly adaptable PIP2 recognition site of talin and potentially other PIP2-binding mechano-proteins.

Automated summary

Focal adhesions (FAs) play a crucial role in mediating the interaction between the cytoskeleton and extracellular matrix. Talin is a key regulator and mechano-sensor of FA complexes. Through molecular dynamics simulations, we discovered that an unstructured loop of the FERM domain of talin-1 interacts strongly with phosphatidylinositol 4,5-bisphosphate (PIP2) membrane, serving as a flexible membrane anchor that facilitates membrane contacts. We identified key residues that mediate the dynamic talin-membrane interaction, suggesting that the intrinsically disordered loop is a key recognition site for talin and potentially other PIP2-binding mechano-proteins.