Structure-activity relationship analysis of activity-based probes targeting HTRA family of serine proteases

High temperature requirement A serine proteases (HTRA) are ubiquitously expressed and participate in protein quality control and cellular stress responses. They are linked to several clinical illnesses, including bacterial infection, cancer, age-related macular degeneration, and neurodegenerative diseases. In addition, several recent studies have revealed HTRAs as important biomarkers and potential therapeutic targets, necessitating the development of an effective detection method to evaluate their functional states in various disease models. We developed a new series of HTRA-targeting activity-based probes with enhanced subtype selectivity and reactivity. In conjunction with our previously developed tetrapeptide probes, we established the structure-activity relationship of the new probes for different HTRA subtypes. Our probes are cell-permeable and have potent inhibitory effects against HTRA1 and HTRA2, making them valuable for identifying and validating HTRAs as an important biomarker.

Automated summary

HTRA is a serine protease that participates in protein quality control and cellular stress responses, and is associated with several clinical disorders. Recent studies have identified HTRA as an important biomarker and potential therapeutic target, necessitating the development of an effective detection method. We have developed a series of HTRA-targeting activity-based probes with enhanced subtype selectivity and reactivity. These probes are cell-permeable and inhibit HTRA1 and HTRA2, making them valuable for identifying and validating HTRAs as important biomarkers.