Exogenous application of melatonin alleviates the drought stress by regulating the antioxidant systems and sugar contents in sorghum seedlings

Drought stress (DS) is a considerable limiting factor that reduces growth and crop productivity. Melatonin (MT) is one of the emerging plant hormones involved in improving drought tolerance in plants. Therefore, the current study was comprised of various MT levels i.e., control, 100, 200, and 300 mu M factorially combined with four sorghum genotypes: YSS-1, YSS-15, Johr, and PARC-S-4. The DS was imposed at the initiation of booting (Zadoks growth scale) by withholding irrigation for 1 week. Taller plants (74.7 cm), with more leaves per plant (LLP: 12), flag leaf area (FLA: 153.7 cm2), root and shoot ratio (0.16), biomass yield (89.1 g), Brix value (9.7%) and soluble solids (SS: 13.3) were recorded with the application of 300 mu M MT. In the case of cultivars YSS-1 performed with maximum plant height (74.7 cm), LPP (11), FLA (143.9 cm2), root and shoot ratio (0.14), biomass yield (87.2 g), Brix value (10.3%) and SS (11.5). The activities of antioxidants (CAT, POD, and SOD) were also significantly increased after the imposition of DS, nonetheless, the application of MT further enhanced the activities of these antioxidants to confer drought tolerance. Therefore, these findings suggested that foliar spray of MT (300 mu M) could alleviate the negative impacts of DS and improve the growth, biomass yield, and nutritional quality of sorghum.

Automated summary

Drought stress is a major limiting factor for crop growth and productivity. Melatonin, as an emerging plant hormone, can improve drought tolerance in plants. This study found that the application of 300 μM melatonin promoted the growth, increased yield, improved nutritional quality, and enhanced antioxidant activity in sorghum plants.