MiR-24-1-5p Hinders Malignant Phenotypes of Clear Cell Renal Cell Carcinoma by Targeting SHOX2

MiRNAs are essential epigenetic modulators that can regulate protein expression. According to the principle of base complementary pairing, miRNA is partially or completely complementary to the 3'-UTR region of its target gene, by which it inhibits the translation of the targeted gene. This study investigated the role of miR-24-1-5p in clear cell renal cell carcinoma (ccRCC). Data in TCGA-KIRC denoted that miR-24-1-5p was under-expressed in ccRCC. Bioinformatics analysis predicted that its target gene was SHOX2, which was significantly expressed in cancer tissues. Dual luciferase assay verified the targeting relationship between miR-24-1-5p and SHOX2. Cell function experiments demonstrated that overexpression of miR-24-1-5p significantly inhibited SHOX2 level and the malignant phenotypes of ccRCC cells. The above results illustrated that miR-24-1-5p/SHOX2 axis was critical for the oncogenesis and development of ccRCC, which might be helpful for us to understand the mechanism and novel therapeutic methods of ccRCC.

Automated summary

MiRNAs are important epigenetic modulators regulating protein expression. This study investigated the role of miR-24-1-5p in clear cell renal cell carcinoma (ccRCC). The study found that miR-24-1-5p was under-expressed in ccRCC and its target gene SHOX2 was significantly expressed in cancer tissues. Functional experiments demonstrated that overexpression of miR-24-1-5p inhibited SHOX2 level and malignant phenotypes of ccRCC cells. These results highlight the critical role of the miR-24-1-5p/SHOX2 axis in ccRCC development, providing potential insights into the mechanism and therapeutic strategies for ccRCC.