Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 657, Issue -, Pages 86-91Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2023.03.054
Keywords
AFM; HaCaT E5/E6/E7-18; Cell mechanics; Pre-tumoral model
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During tumorigenesis, the mechanical properties of cancer cells change, with decreased stiffness often accompanying a more invasive phenotype. A recent study used atomic force microscopy to measure cell stiffness and observed a decrease in Young's modulus and cell rigidity in the pre-tumoral cell model of cervical cancer. This study suggests that decreased stiffness and perturbation in cell shape are early mechanical and morphological changes during malignant transformation.
During tumorigenesis, the mechanical properties of cancer cells change markedly, with decreased stiffness often accompanying a more invasive phenotype. Less is known about the changes in mechanical parameters at intermediate stages in the process of malignant transformation. We have recently developed a pre-tumoral cell model by stably transducing the immortalized but non-tumorigenic human keratinocyte cell line HaCaT with the E5, E6 and E7 oncogenes from HPV-18, one of the leading causes of cervical cancer and other types of cancer worldwide. We have used atomic force microscopy (AFM) to measure cell stiffness and to obtain mechanical maps of parental HaCaT and HaCaT E5/E6/E7-18 cell lines. We observed a significant decrease in Young's modulus in HaCaT E5/E6/E7-18 cells measured by nanoindentation in the central region, as well as decreased cell rigidity in regions of cell-cell contact measured by Peakforce Quantitative Nanomechanical Mapping (PF-QNM). As a morphological correlate, HaCaT E5/E6/E7-18 cells displayed a significantly rounder cell shape than parental HaCaT cells. Our re-sults therefore show that decreased stiffness with concomitant perturbations in cell shape are early mechanical and morphological changes during the process of malignant transformation.& COPY; 2023 Elsevier Inc. All rights reserved.
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