Effects of bisphenol F, bisphenol S, and bisphenol AF on cultured human osteoblasts

Bisphenol A (BPA) analogs, like BPA, could have adverse effects on human health including bone health. The aim was to determine the effect of BPF, BPS and BPAF on the growth and differentiation of cultured human osteoblasts. Osteoblasts primary culture from bone chips harvested during routine dental work and treated with BPF, BPS, or BPAF for 24 h at doses of 10(-5), 10(-6), and 10(-7) M. Next, cell proliferation was studied, apoptosis induction, and alkaline phosphatase (ALP) activity. In addition, mineralization was evaluated at 7, 14, and 21 days of cell culture in an osteogenic medium supplemented with BP analog at the studied doses. BPS treatment inhibited proliferation in a dose-dependent manner at all three doses by inducing apoptosis; BPF exerted a significant inhibitory effect on cell proliferation at the highest dose alone by an increase of apoptosis; while BPAF had no effect on proliferation or cell viability. Cell differentiation was adversely affected by treatment with BPA analogs in a dose-dependent, observing a reduction in calcium nodule formation at 21 days. According to the results obtained, these BPA analogs could potentially pose a threat to bone health, depending on their concentration in the organism.

Automated summary

The aim of this study was to determine the effects of BPF, BPS, and BPAF on the growth and differentiation of cultured human osteoblasts. The results showed that BPS inhibited cell proliferation in a dose-dependent manner by inducing apoptosis; BPF significantly inhibited cell proliferation at the highest dose alone by increasing apoptosis; while BPAF had no effect on proliferation or cell viability. Furthermore, treatment with BPA analogs adversely affected cell differentiation, as evidenced by a reduction in calcium nodule formation at 21 days. These findings suggest that these BPA analogs could potentially pose a threat to bone health depending on their concentration in the body.