Inorganic polyphosphate's role in energy production and mitochondrial permeability transition pore opening in tick mitochondria

Inorganic polyphosphate (polyP) is a biopolymer composed of phosphoanhydride-linked orthophosphate molecules. PolyP is engaged in a variety of cellular functions, including mitochondrial metabolism. Here, we examined the effects of polyP on electron transport chain enzymes and F1Fo ATP synthase in tick embryos during embryonic development. The study found that polyPs containing medium and long chains (polyP(15) and polyP(65)) enhanced the activity of complex I, complex II, complex III, and F1Fo ATP synthase, while short polyP chains (polyP(3)) had no effect. The study also examined the activity of exopolyphosphatases (PPX) in various energy-demand situations. PPX activity was stimulated when ADP concentrations are high, characterizing a low-energy context. When complexes I-III and F1Fo ATP synthase inhibitors were added in energized mitochondria, PPX activity decreased, whereas the mitochondrial uncoupler FCCP had no impact on PPX activity. Additionally, the study investigated the effect of polyP on mitochondrial swelling, finding that polyP causes mitochondrial swelling by increasing calcium effects on the mitochondrial permeability transition pore. The findings presented here to increase our understanding of the function of polyP in mitochondrial metabolism and its relationship to mitochondrial permeability transition pore opening in an arthropod model.

Automated summary

This study found that polyP enhances the activity of electron transport chain enzymes and F1Fo ATP synthase in tick embryos during embryonic development. Long and medium polyP chains (polyP(15) and polyP(65)) increased the activity of complex I, complex II, complex III, and F1Fo ATP synthase, while short polyP chains (polyP(3)) had no effect. The study also demonstrated that PPX activity is stimulated in low-energy conditions and is decreased when inhibitors of complexes I-III and F1Fo ATP synthase are added in energized mitochondria. Furthermore, polyP causes mitochondrial swelling by increasing the effects of calcium on the mitochondrial permeability transition pore.