Formation of neuron-microelectrode junction mediated by a synapse organizer

Neural circuits are composed of various cell types, each of which is thought to play a specific role in realizing the circuit functions. Cell-type specificity is therefore essential in recording neuronal activities but is inherently lacking in the currently available microelectrode techniques. Here, using Interleukin-1 receptor accessory protein-like 1 (IL1RAPL1) as a model synapse organizer, we show a proof-of-principle experiment that a microelectrode functionalized with a synapse organizer is capable of inducing a synapse-like junction between a neuron upon physical contact. We then discuss prospects for developing a new mode of electrophysiology that permits cell-type specific recordings via such a molecularly induced neuron-microelectrode junction.

Automated summary

Neural circuits consist of different cell types, each playing a specific role in circuit functions. Current microelectrode techniques lack cell-type specificity in recording neuronal activities. This study demonstrates a proof-of-principle experiment using IL1RAPL1 as a model synapse organizer, showing that a microelectrode functionalized with a synapse organizer can induce a synapse-like junction between neurons upon physical contact. The prospects of developing a new mode of electrophysiology that allows cell-type specific recordings via molecularly induced neuron-microelectrode junctions are then discussed.