Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 107, Issue 12, Pages 3967-3981Publisher
SPRINGER
DOI: 10.1007/s00253-023-12561-w
Keywords
alpha-L-Arabinofuranosidases; Trametes hirsuta; Arabinoxylan; Synergistic degradation; Glycoside hydrolase 51 family; Crystal structure
Categories
Ask authors/readers for more resources
In this study, an arabinofuranosidase (ThAbf1) belonging to the glycoside hydrolase 51 (GH51) family was expressed, characterized, and functionally determined from the genome of the white-rot fungus Trametes hirsuta. It was found that ThAbf1 synergized with commercial xylanase (XYL) and increased the saccharification efficiency of arabinoxylan.
a-L-Arabinofuranosidases (Abfs) play a crucial role in the degradation of hemicelluloses, especially arabinoxylans (AX). Most of the available characterized Abfs are from bacteria, while fungi, as natural decomposers, contain Abfs with little attention given. An arabinofuranosidase (ThAbf1), belonging to the glycoside hydrolase 51 (GH51) family, from the genome of the white-rot fungus Trametes hirsuta, was recombinantly expressed, characterized, and functionally determined. The general biochemical properties showed that the optimal conditions for ThAbf1 were pH 6.0 and 50?. In substrate kinetics assays, ThAbf1 preferred small fragment arabinoxylo-oligosaccharides (AXOS) and could surprisingly hydrolyze di-substituted 2(3),3(3)-di-L-arabinofuranosyl-xylotriose (A(2,3)XX). It also synergized with commercial xylanase (XYL) and increased the saccharification efficiency of arabinoxylan. The crystal structure of ThAbf1 indicated the presence of an adjacent cavity next to the catalytic pocket which led to the ability of ThAbf1 to degrade di-substituted AXOS. The narrow binding pocket prevents ThAbf1 from binding larger substrates. These findings have strengthened our understanding of the catalytic mechanism of GH51 family Abfs and provided a theoretical foundation for the development of more efficient and versatile Abfs to accelerate the degradation and biotransformation of hemicellulose in biomass.{Graphical abstract}
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available