4.8 Article

Directed Evolution of Piperazic Acid Incorporation by a Nonribosomal Peptide Synthetase

Related references

Note: Only part of the references are listed.
Article Chemistry, Multidisciplinary

Free Piperazic Acid as a Precursor to Nonribosomal Peptides

Zi-Wang Wei et al.

Summary: Piperazic acid is incorporated into nonribosomal peptides through adenylation, and the consensus sequences for its adenylation have been determined. This study provides valuable insights into the biosynthesis and function of Piperazic acid.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2022)

Article Chemistry, Multidisciplinary

Reprogramming Nonribosomal Peptide Synthetases for Site-Specific Insertion of α-Hydroxy Acids

Anna Camus et al.

Summary: This study demonstrates the successful switch of substrate specificity in gatekeeper adenylation domains of nonribosomal peptide synthetases using high-throughput engineering, leading to efficient production of diverse peptide metabolites.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2022)

Article Multidisciplinary Sciences

Structures of a non-ribosomal peptide synthetase condensation domain suggest the basis of substrate selectivity

Thierry Izore et al.

Summary: Non-ribosomal peptide synthetases (NRPSs) are essential for assembling complex natural products, with condensation domains playing a central role in chain assembly. Structural snapshots of a condensation domain bound to an aminoacyl-PCP substrate reveal a mechanism for controlling substrate access and tuning selectivity, without requiring a distinct pocket for side chain selection during peptide assembly.

NATURE COMMUNICATIONS (2021)

Review Chemistry, Multidisciplinary

Directed Evolution: Methodologies and Applications

Yajie Wang et al.

Summary: Directed evolution aims to accelerate the natural evolution process of biological molecules and systems through gene diversification and library screening, serving as a powerful tool for engineering improved functions in proteins, metabolic pathways, and whole genomes. Common strategies include gene diversification, screening/selection methods, and continuous evolution, with applications in nucleic acids, proteins, pathways, genetic circuits, viruses, and cells. Challenges and future perspectives in directed evolution are also discussed.

CHEMICAL REVIEWS (2021)

Article Chemistry, Physical

Engineered Nonribosomal Peptide Synthetase Shows Opposite Amino Acid Loading and Condensation Specificity

Aleksa Stanisic et al.

Summary: Engineering of nonribosomal peptide synthetases (NRPSs) faces challenges, with specificity filters determining success and debate over the contribution of adenylation (A) and condensation (C) domains. Kinetic modeling shows that product specificity changes during reactions, with the A-domain overriding C-domain specificity when the thiolation domain loading reaches a steady state. This nonlinear modeling provides critical insights into how the interplay of the A- and C-domains determines product specificity in NRPSs.

ACS CATALYSIS (2021)

Review Chemistry, Multidisciplinary

The Crucial Role of Methodology Development in Directed Evolution of Selective Enzymes

Ge Qu et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020)

Article Multidisciplinary Sciences

Efficient rational modification of non-ribosomal peptides by adenylation domain substitution

Mark J. Calcott et al.

NATURE COMMUNICATIONS (2020)

Review Biotechnology & Applied Microbiology

Recent Advances in Re-engineering Modular PKS and NRPS Assembly Lines

Charlotte Beck et al.

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING (2020)

Article Chemistry, Multidisciplinary

Exploring modular reengineering strategies to redesign the teicoplanin non-ribosomal peptide synthetase

Milda Kaniusaite et al.

CHEMICAL SCIENCE (2020)

Review Biotechnology & Applied Microbiology

Targeting adenylate-forming enzymes with designed sulfonyladenosine inhibitors

Michaelyn C. Lux et al.

JOURNAL OF ANTIBIOTICS (2019)

Review Microbiology

Engineering enzymatic assembly lines to produce new antibiotics

Kenan A. J. Bozhuyuk et al.

CURRENT OPINION IN MICROBIOLOGY (2019)

Article Biotechnology & Applied Microbiology

Structural basis of the nonribosomal codes for nonproteinogenic amino acid selective adenylation enzymes in the biosynthesis of natural products

Fumitaka Kudo et al.

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY (2019)

Review Biochemistry & Molecular Biology

Nonribosomal antibacterial peptides that target multidrug-resistant bacteria

Yuan Liu et al.

NATURAL PRODUCT REPORTS (2019)

Review Biochemistry & Molecular Biology

Piperazic acid-containing natural products: structures and biosynthesis

Kalindi D. Morgan et al.

NATURAL PRODUCT REPORTS (2019)

Article Chemistry, Multidisciplinary

De novo design and engineering of non-ribosomal peptide synthetases

Kenan A. J. Bozhueyuek et al.

NATURE CHEMISTRY (2018)

Article Chemistry, Multidisciplinary

Nonribosomal biosynthesis of backbone-modified peptides

David L. Niquille et al.

NATURE CHEMISTRY (2018)

Article Chemistry, Multidisciplinary

Directed Evolution: Bringing New Chemistry to Life

Frances H. Arnold

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018)

Article Biochemistry & Molecular Biology

A heme-dependent enzyme forms the nitrogen-nitrogen bond in piperazate

Yi-Ling Du et al.

NATURE CHEMICAL BIOLOGY (2017)

Review Chemistry, Multidisciplinary

Heteroatom-Heteroatom Bond Formation in Natural Product Biosynthesis

Abraham J. Waldman et al.

CHEMICAL REVIEWS (2017)

Review Chemistry, Multidisciplinary

Nonribosomal Peptide Synthesis-Principles and Prospects

Roderich D. Suessmuth et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2017)

Review Biochemistry & Molecular Biology

Recent advances in engineering nonribosomal peptide assembly lines

M. Winn et al.

NATURAL PRODUCT REPORTS (2016)

Article Multidisciplinary Sciences

Structures of two distinct conformations of holo-non-ribosomal peptide synthetases

Eric J. Drake et al.

NATURE (2016)

Article Chemistry, Multidisciplinary

Structural Elucidation of the Bispecificity of A Domains as a Basis for Activating Non-natural Amino Acids

Heidi Kaljunen et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2015)

Article Biochemistry & Molecular Biology

A Subdomain Swap Strategy for Reengineering Nonribosomal Peptides

Hajo Kries et al.

CHEMISTRY & BIOLOGY (2015)

Review Genetics & Heredity

Methods for the directed evolution of proteins

Michael S. Packer et al.

NATURE REVIEWS GENETICS (2015)

Article Chemistry, Multidisciplinary

Reprogramming Nonribosomal Peptide Synthetases for Clickable Amino Acids

Hajo Kries et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2014)

Article Biotechnology & Applied Microbiology

Biosynthesis of Novel Pyoverdines by Domain Substitution in a Nonribosomal Peptide Synthetase of Pseudomonas aeruginosa

Mark J. Calcott et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2014)

Article Biochemistry & Molecular Biology

The Crystal Structure of the Adenylation Enzyme VinN Reveals a Unique β-Amino Acid Recognition Mechanism

Akimasa Miyanaga et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2014)

Article Biochemistry & Molecular Biology

Engineering the Substrate Specificity of the DhbE Adenylation Domain by Yeast Cell Surface Display

Keya Zhang et al.

CHEMISTRY & BIOLOGY (2013)

Article Chemistry, Multidisciplinary

Introduction of a Non-Natural Amino Acid into a Nonribosomal Peptide Antibiotic by Modification of Adenylation Domain Specificity

Jenny Thirlway et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2012)

Article Biochemistry & Molecular Biology

Biosynthesis of Piperazic Acid via N5-Hydroxy-Ornithine in Kutzneria spp. 744

Christopher S. Neumann et al.

CHEMBIOCHEM (2012)

Article Chemistry, Medicinal

Polycationic Gramicidin S Analogues with Both High Antibiotic Activity and Very Low Hemolytic Activity

Makoto Tamaki et al.

CHEMICAL & PHARMACEUTICAL BULLETIN (2012)

Article Biochemistry & Molecular Biology

Directed Evolution of a Gatekeeper Domain in Nonribosomal Peptide Synthesis

Benoit Villiers et al.

CHEMISTRY & BIOLOGY (2011)

Article Biochemistry & Molecular Biology

Directed Evolution of the Nonribosomal Peptide Synthetase AdmK Generates New Andrimid Derivatives In Vivo

Bradley S. Evans et al.

CHEMISTRY & BIOLOGY (2011)

Review Biochemistry & Molecular Biology

Piperazic acid-containing natural products: Isolation, biological relevance and total synthesis

Alexander J. Oelke et al.

NATURAL PRODUCT REPORTS (2011)

Article Biochemistry & Molecular Biology

NRPSpredictor2-a web server for predicting NRPS adenylation domain specificity

Marc Roettig et al.

NUCLEIC ACIDS RESEARCH (2011)

Article Biochemical Research Methods

A continuous kinetic assay for adenylation enzyme activity and inhibition

Daniel J. Wilson et al.

ANALYTICAL BIOCHEMISTRY (2010)

Article Multidisciplinary Sciences

Cloning and characterization of the biosynthetic gene cluster for kutznerides

Danica Galonic Fujimori et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2007)

Article Multidisciplinary Sciences

Directed evolution can rapidly improve the activity of chimeric assembly-line enzymes

Michael A. Fischbach et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2007)

Review Chemistry, Multidisciplinary

Molecular mechanisms underlying nonribosomal peptide synthesis: Approaches to new antibiotics

SA Sieber et al.

CHEMICAL REVIEWS (2005)