Microfluidic Gradient Culture Arrays for Cell Pro-oxidation Analysis Using Bipolar Electrochemiluminescence

A microfluidic gradient array isa widely used screening and analysisdevice, which has characteristics of high efficiency, high automation,and low consumption. Bipolar electrode electrochemiluminescence (BPE-ECL)has special value in microfluidic array chips. The combination ofthe microfluidic gradient and BPE arrays has potential for high-throughputscreening. In this article, a microfluidic BPE array chip for gradientculture and conditional screening of cancer cells was designed. Thegeneration of concentration gradients, continuous culture of cancercells with high throughput, and drug screening through BPE-ECL ofthe Ru-(bpy)(3) (2+)/TPrA system can be performedin one chip. We tested gradient pro-oxidation of MCF-7 by ascorbicacid and the synergistic effect of pro-oxidation on doxorubicin. Themethod achieves high analysis efficiency through a BPE array whilesimplifying the tedious procedures required by cell culture methods.

Automated summary

In this article, a microfluidic BPE array chip was designed for gradient culture and conditional screening of cancer cells. The chip enables the generation of concentration gradients, continuous high-throughput culture of cancer cells, and drug screening through BPE-ECL of the Ru-(bpy)(3)(2+)/TPrA system. The use of the BPE array simplifies the tedious procedures required by cell culture methods and achieves high analysis efficiency.