Journal
ANALYTICAL CHEMISTRY
Volume 95, Issue 32, Pages 11885-11891Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.3c00143
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In this study, a novel IMNs platform was developed by biomimetic protein corona precoating and polyethylene glycol (PEG) spacer, forming PEG and corona-coated IMNs (IP-CMNs). The IP-CMNs demonstrated a dual stealth effect, reducing nonspecific protein adsorption and cell binding, and enhancing capture performance towards tumor cells.
Asa biomarker of hepatocellular carcinoma (HCC) biopsy, circulatingtumor cells (CTCs) are often used in the diagnosis of cancer and treatmentguidance. For CTCs detection, immuno-magnetic nanoparticles (IMNs)are one of the most commonly used platforms. However, the nonspecificadsorption of proteins and non-tumor cells weakens the performanceof IMNs to capture CTCs. In this work, we developed an IMNs platformwhich was constructed by a biomimetic protein corona precoating anda polyethylene glycol (PEG) spacer to form the PEG and corona-coatedIMNs (IP-CMNs). Due to the dual stealth effect of protein corona precoatingand PEG spacer, the nonspecific protein adsorption and cell bindingof P-CMNs could reduce by & SIM;5.5- and & SIM;5.4-fold, respectively,compared with those of unmodified particles. Furthermore, the PEGspacer could not only reduce the interaction between IP-CMNs and leukocytesbut also enhance the capture performance toward tumor cells. By usingartificial blood samples, the capture efficiency of IP-CMNs towardrare CTCs was found to be 88.3%, while it was 70.5% by using commercialIMNs. Finally, CTCs were successfully isolated in all HCC patientblood samples (7/7) using IP-CMNs. These results provide insight intothe use of the multifunctional nanoplatform as a useful tool for CTCsdetection.
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