Encapsulation of Tetraphenylethylene Derivative in Liposome Vesicles as Promising Aggregation-Induced Electrochemiluminescence Emitter for Detection of Human Epidermal Growth Factor Receptor 2

In this work, a sensitive signal-on electrochemiluminescencebiosensorusing liposome-encapsuled 1,1,2,2-tetra(4-carboxylphenyl)ethylene(TPE) as a promising aggregation-induced electrochemiluminescence(AIECL) emitter for detection of biomarkers was developed. Aggregation-inducedenhancement occurs internally through the spatial confinement effectand intramolecular self-encapsulation of encapsulating TPE and triethylamine(TEA) molecules in liposome cavities. Peptide sequence WTGW-CLNP-EEST-WGFC-TGSF(WF-20) was used to replace the antibody for reducing the steric hindranceof the sensing surface while taking into account the affinity. Theproposed sensing strategies showed satisfactory properties for detectionof human epidermal growth factor receptor 2 (HER2) ranging from 0.01to 500 ng/mL with a limit of detection of 6.65 pg/mL. The resultsconfirmed that encapsulation of luminescent molecules in the vesiclestructure for triggering the AIECL phenomenon is a promising methodto prepare a signal label for a trace detection biomarker.

Automated summary

A sensitive signal-on electrochemiluminescence biosensor using liposome-encapsulated 1,1,2,2-tetra(4-carboxylphenyl)ethylene (TPE) as an aggregation-induced electrochemiluminescence (AIECL) emitter for biomarker detection was developed. The proposed sensing strategy utilized the spatial confinement effect and intramolecular self-encapsulation of TPE and triethylamine (TEA) molecules in liposome cavities for aggregation-inducedenhancement. The peptide sequence WTGW-CLNP-EEST-WGFC-TGSF (WF-20) was used to replace the antibody for reduced steric hindrance on the sensing surface while maintaining affinity. The developed biosensor showed satisfactory properties for the detection of human epidermal growth factor receptor 2 (HER2), with a detection range of 0.01 to 500 ng/mL and a limit of detection of 6.65 pg/mL.