Journal
ANALYTICAL CHEMISTRY
Volume 95, Issue 14, Pages 5946-5954Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.2c05473
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This research developed a DNA-mediated self-assembly technology to achieve efficient and accurate quantification of lactoferrin (Lac) and iron ions (Fe3+) in human serum and milk samples, providing valuable insights for human health and early diagnosis of anemia.
DNA-mediated self-assembly technology with good sensitivity and affinity ability has been rapidly developed in the field of probe sensing. The efficient and accurate quantification of lactoferrin (Lac) and iron ions (Fe3+) in human serum and milk samples by the probe sensing method can provide useful clues for human health and early diagnosis of anemia. In this paper, contractile hairpin DNA-mediated dual-mode probes of Fe3O4/Ag-ZIF8/graphitic quantum dot (Fe3O4/Ag-ZIF8/GQD) NPs were prepared to realize the simultaneous quantification of Lac by surface-enhanced Raman scattering (SERS) and Fe3+ by fluorescence (FL). In the presence of targets, these dual-mode probes would be triggered by the recognition of aptamer and release GQDs to produce FL response. Meanwhile, the complementary DNA began to shrink and form a new hairpin structure on the surface of Fe3O4/Ag, which produced hot spots and generated a good SERS response. Thus, the proposed dual-mode analytical strategy possessed excellent selectivity, sensitivity, and accuracy due to the dual-mode switchable signals from off to on in SERS mode and from on to offin FL mode. Under the optimized conditions, a good linear range was obtained in the range of 0.5-100.0 mu g/ L for Lac and 0.01-5.0 mu mol/L for Fe3+ and with detection limits of 0.14 mu g/L and 3.8 nmol/L, respectively. Finally, the contractile hairpin DNA-mediated SERS-FL dual-mode probes were successfully applied in the simultaneous quantification of iron ion and Lac in human serum and milk samples.
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