Journal
ANALYTICAL CHEMISTRY
Volume 95, Issue 20, Pages 7822-7829Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.2c04817
Keywords
-
Categories
Ask authors/readers for more resources
Hyperpolarization modalities, particularly SABRE, are employed to enhance 15N polarization of nitrile-containing anticancer drugs. Optimal parameters, including temperature, magnetic field, and field profile, are fine-tuned for different substrates. Over 10% hyperpolarization and 280,000-fold signal enhancement are achieved for letrozole and anastrozole. Prolonged polarization storage and signal detection are enabled by the nitrile 15N sites.
Hyperpolarization modalities overcome the sensitivity limitations of NMR and unlock new applications. Signal amplification by reversible exchange (SABRE) is a particularly cheap, quick, and robust hyperpolarization modality. Here, we employ SABRE for simultaneous chemical exchange of parahydrogen and nitrile-containing anticancer drugs (letrozole or anastrozole) to enhance 15N polarization. Distinct substrates require unique optimal parameter sets, including temperature, magnetic field, or a shaped magnetic field profile. The fine tuning of these parameters for individual substrates is demonstrated here to maximize 15N polarization. After optimization, including the usage of pulsed mu T fields, the 15N nuclei on common anticancer drugs, letrozole and anastrozole, can be polarized within 1-2 min. The hyperpolarization can exceed 10%, corresponding to 15N signal enhancement of over 280,000-fold at a clinically relevant magnetic field of 1 T. This sensitivity gain enables polarization studies at naturally abundant 15N enrichment level (0.4%). Moreover, the nitrile 15N sites enable long-lasting polarization storage with [15N]T1 over 9 min, enabling signal detection from a single hyperpolarization cycle for over 30 min.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available