Journal
ANALYTICAL CHEMISTRY
Volume 95, Issue 17, Pages 7045-7052Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.3c00729
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A novel sandwich electrochemiluminescence biosensor was developed for the sensitive detection of Afl42 using aggregation-induced electrochemiluminescence resonance energy transfer. The biosensor utilized silver nanoparticle-functionalized zinc metal-organic framework as the ECL donor and gold nanoparticle-functionalized zirconium organic framework as the acceptor. The method exhibited a linear detection range of 10 fg/mL to 100 ng/mL and a detection limit of 2.4 fg/mL. It also demonstrated good stability, repeatability, and specificity, making it a promising tool for disease biomarker detection.
A novel sandwich electrochemiluminescence (ECL) biosensor based on aggregation-induced electrochemiluminescence resonance energy transfer (AIECL-RET) was designed for the sensitive detection of amyloid fl42 (Afl42). The synthesized silver nanoparticle-functionalized zinc metal-organic framework (Ag@ ZnPTC) and gold nanoparticle-functionalized zirconium organic framework (Au@UiO-66-NH2) were used as the ECL donor and acceptor, respectively. AgNPs were generated in situ on the surface of ZnPTC, which further improved the ECL intensity and the loading of antibody 1 (Ab1). Under the optimized experimental conditions, the linear detection range of Afl42 concentration was 10 fg/mL to 100 ng/mL, and the detection limit was 2.4 fg/mL (S/N = 3). The recoveries of Afl42 were 99.5-104%. The method has good stability, repeatability, and specificity. Ag@ZnPTC/Au@UiO-66-NH2 provides an assay for the sensitive detection of disease biomarkers.
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