Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 138, Issue 43, Pages 14497-14502Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jacs.6b09545
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Funding
- National Basic Research Program of China (973 program) [2013CB932800]
- NSFC [21532004, 81621002, 21621003]
- Chinese Ministry of Science and Technology [2015CB910104, 2016YFA0501103]
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Racemic or quasi-racemic crystallography recently emerges as a useful technology for solution of the crystal structures of biomacromolecules. It remains unclear to what extent the biomacromolecules of opposite handedness can differ from each other in racemic or quasi-racemic crystallography. Here we report a finding that monomeric D-ubiquitin (Ub) has propensity to cocrystallize with different dimers, trimers, and even a tetramer of L-Ub. In these cocrystals the unconnected monomeric D-Ubs can self-assemble to form pseudomirror images of different oligomers of L-Ub. This monomer/oligomer cocrystallization phenomenon expands the concept of racemic crystallography. Using the monomer/oligomer cocrystallization technology we obtained, for the first time the X-ray structures of linear M1-linked tri- and tetra-Ubs and a K11/K63-branched tri-Ub.
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