Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 138, Issue 8, Pages 2476-2479Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jacs.5b10939
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Funding
- Ontario Research Fund
- Natural Sciences and Engineering Council of Canada
- Canadian Institutes for Health Research
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Cancer cells, and in particular those found circulating in blood, can have widely varying phenotypes and molecular profiles despite a common origin. New methods are needed that can deconvolute the heterogeneity of cancer cells and sort small numbers of cells to aid in the characterization of cancer cell subpopulations: Here, we describe a new molecular approach to capturing cancer cells that isolates subpopulations using two-dimensional sorting. Using aptamer-mediated capture and antisense-triggered release, the new strategy sorts cells according to levels of two different markers and thereby sepatates them into their corresponding sub populations. Using a phenotypic assay) we demonstrate that the subpopulations isolated have markedly different properties. This system provides an important new tool for identifying circulating tumor cell subtypes.
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