Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 139, Issue 1, Pages 111-114Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jacs.6b12297
Keywords
-
Categories
Funding
- EU Marie Curie PATHCHOOSER project [PITN-GA-2013-608373]
- EU FP7 FutureNanoNeeds project [604602]
- Science Foundation Ireland (SFI) [12/IA/1422]
- Science Foundation Ireland (SFI) [12/IA/1422] Funding Source: Science Foundation Ireland (SFI)
Ask authors/readers for more resources
Characterizing the orientation of covalently conjugated proteins on nanoparticles, produced for in vitro and in vivo targeting, though an important feature of such a system, has proved challenging. Although extensive physicochemical characterization of targeting nanoparticles can be addressed in detail, relevant biological characterization of the nanointerface is crucial in order to select suitable nanomaterials for further in vitro or in vivo experiments. In this work, we adopt a methodology using antibody fragments (Fab) conjugated to gold nanoparticles (immunogold) to map the available epitopes on a transferrin grafted silica particle (SiO2-PEG(8)-Tf) as a proxy methodology to predict nanoparticle biological function, and therefore cellular receptor engagement. Data from the adopted method suggest that, on average, only similar to 3.5% of proteins grafted on the SiO2-PEG(8)-Tf nanoparticle surface have a favorable orientation for recognition by the cellular receptor.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available