Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 138, Issue 25, Pages 7852-7855Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jacs.6b04491
Keywords
-
Categories
Funding
- NIH [GM059636, GM107820]
- Commonwealth of Kentucky Research Challenge Trust Fund
- Center for Regulatory and Analytical Metabolomics (CREAM)
- NSF/EPSCoR [EPS-0447479]
Ask authors/readers for more resources
The pseudouridine synthases isomerize (U) in RNA to pseudouridine (Psi), and the mechanism that they follow has long been a question of interest. The recent elucidation of a product of the mechanistic probe 5-fluorouridine that had been epimerized to the arabino isomer suggested that the Psi synthases might operate through a glycal intermediate formed by deprotonation of C2'. When that position in substrate U is deuterated, a primary kinetic isotope effect is observed, which indisputably indicates that the proposed deprotonation occurs during the isomerization of U to Psi and establishes the mechanism followed by the Psi synthases.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available