A Facile and Versatile Approach to Construct Photoactivated Peptide Hydrogels by Regulating Electrostatic Repulsion

Short peptides that can respond to external stimuli have been considered as the preferred building blocks to construct hydrogels for biomedical applications. In particular, photoresponsive peptides that are capable of triggering the formation of hydrogels upon light irradiation allow the properties of hydrogels to be changed remotely by precise and localized actuation. Here, we used the photo-chemical reaction of the 2-nitrobenzyl ester group (NB) to develop a facile and versatile strategy for constructing photoactivated peptide hydrogels. The peptides with high aggregation propensity were designed as hydrogelators, which were photocaged by a positively charged dipeptide (KK) to provide strong charge repulsion and prevent self-assembly in water. Light irradiation led to the removal of KK and triggered the self-assembly of peptides and the formation of hydrogel. Light stimulation endows spatial and temporal control, which enables the formation of hydrogel with precisely tunable structure and mechanical properties. Cell culture and behavior study indicated that the optimized photoactivated hydrogel was suitable for 2D and 3D cell culture, and its photocontrollable mechanical strength could regulate the spreading of stem cells on its surface. Therefore, our strategy provides an alternative way to construct photoactivated peptide hydrogels with wide applications in biomedical areas.

Automated summary

Short peptides that respond to external stimuli have been utilized to construct hydrogels for biomedical applications. In this study, a photoactivated peptide hydrogel was developed using a photo-chemical reaction and a positively charged dipeptide. Light irradiation triggered the self-assembly of peptides and the formation of hydrogel, allowing precise control over its structure and mechanical properties. The optimized photoactivated hydrogel was suitable for cell culture and showed photocontrollable mechanical strength that regulated stem cell spreading. This strategy provides an alternative method for constructing photoactivated peptide hydrogels with broad applications in biomedicine.