Dynamic and Rapid Detection of Guanosine during Ischemia

Guanosine acts in both neuroprotective and neurosignaling pathways in the central nervous system; in this paper, we present the first fast voltammetric measurements of endogenous guanosine release during pre-and post-ischemic conditions. We discuss the metric of our measurements via analysis of event concentration, duration, and interevent time of rapid guanosine release. We observe changes across all three metrics from our normoxic to ischemic conditions. Pharmacological studies were performed to confirm that guanosine release is a calcium-dependent process and that the signaling observed is purinergic. Finally, we show the validity of our ischemic model via staining and fluorescent imaging. Overall, this paper sets the tone for rapid monitoring of guanosine and provides a platform to investigate the extent to which guanosine accumulates at the site of brain injury, i.e., ischemia.

Automated summary

In this paper, the authors measure endogenous guanosine release using fast voltammetry during pre- and post-ischemic conditions. Changes in event concentration, duration, and interevent time indicate alterations in guanosine release under ischemic conditions. Pharmacological studies confirm the calcium-dependent nature of guanosine release and its purinergic signaling. The validity of the ischemic model is demonstrated through staining and fluorescent imaging. Overall, this paper paves the way for rapid guanosine monitoring and provides a platform for investigating guanosine accumulation at the site of brain injury, such as ischemia.