Ultrasensitive miRNA-21 Biosensor Based on Zn(TCPP) PET-RAFT Polymerization Signal Amplification and Multiple Logic Gate Molecular Recognition

Quantitative measurement of microRNAs (miRNAs) is extremely important in plenty of biomedical applications especially cancer diagnosis but remains a great challenge. In this work, we developed a logic gate recognition biosensing platform based on the trinity molecular recognition mode for quantifying miRNAs with a detection limit of 4.48 aM, along with a linear range from 0.1 nM to 10 aM under optimal experimental conditions. In order to obtain excellent detection performance, we adopted a Zn(TCPP) photocatalytic electron transfer reversible tion signal amplification strategy. The light-induced PET-RAFT has developed green applications of free radical polymerization in the field of biosensors. This is the first report on the preparation of signal amplification biosensors using PET-RAFT for tumor marker detection. With the outstanding detection performance, we can apply the sensor system to the early screening of lung cancer patients.

Automated summary

In this study, a logic gate recognition biosensing platform based on the trinity molecular recognition mode was developed for quantitative measurement of microRNAs (miRNAs), particularly in cancer diagnosis. The platform exhibited high sensitivity and a linear range from 0.1 nM to 10 aM, with a detection limit of 4.48 aM under optimal experimental conditions. A Zn(TCPP) photocatalytic electron transfer reversible tion signal amplification strategy was adopted to achieve excellent detection performance. This study is the first to report the preparation of signal amplification biosensors using PET-RAFT for tumor marker detection. With its outstanding detection performance, the sensor system can be applied to early screening of lung cancer patients.