Construction of the Mussel-Inspired PDAM/Lysine/Heparin Composite Coating Combining Multiple Anticoagulant Strategies

The thrombosis of the extracorporeal circuits leads toseriouscomplications, which affect the life safety of the patients significantly.However, intravenous anticoagulants such as heparin may induce bleeding,hypersensitivity, and other adverse reactions. In this study, themussel-inspired composite coating consisting of polydopamine (PDAM),lysine, and modified heparin was constructed on the commonly usedmedical poly-(vinyl chloride) (PVC) tubes to reduce thrombosis by combiningthe immobilization of anticoagulants and the construction of bioinertsurface strategies. First, the PDAM/lysine coating rich in amine groupswas constructed in a mixed solution of dopamine and lysine throughthe co-deposition reaction. Then, the modified heparin was covalentlyimmobilized on the PDAM/lysine coating to obtain composite coating.Finally, the graft density and stability of heparin and anticoagulantproperties of the composite coating were tested. The results showedthat the composite coating could inhibit the adhesion and activationof platelets significantly and prolong the activated partial thromboplastintime (APTT) remarkably for over 25 s. The composite coating also hadexcellent hemocompatibility, and the hemolysis ratio was less than0.5%. Particularly, the anticoagulant coating performed well in thein vitro blood circulation test. The composite coating constructedin this work show great potential in the anticoagulant treatment formedical devices.

Automated summary

In this study, a mussel-inspired composite coating consisting of polydopamine, lysine, and modified heparin was constructed to reduce thrombosis and enhance the anticoagulant capability of medical devices. The results showed that the composite coating significantly inhibited platelet adhesion and activation, prolonged activated partial thromboplastin time, and exhibited excellent hemocompatibility and anticoagulant properties. This research demonstrates great potential for anticoagulant treatment in medical devices.