Feasibility of Coacervate-Like Nanostructure for Instant Drug Nanoformulation

Despite the enormous advancements in nanomedicine research, a limited number of nanoformulations are available on the market, and few have been translated to clinics. An easily scalable, sustainable, and cost-effective manufacturing strategy and long-term stability for storage are crucial for successful translation. Here, we report a system and method to instantly formulate NF achieved with a nanoscale polyelectrolyte coacervate-like system, consisting of anionic pseudopeptide poly(L-lysine isophthalamide) derivatives, polyethylenimine, and doxorubicin (Dox) via simple mix-and-go addition of precursor solutions in seconds. The coacervate-like nanosystem shows enhanced intracellular delivery of Dox to patient-derived multidrug-resistant (MDR) cells in 3D tumor spheroids. The results demonstrate the feasibility of an instant drug formulation using a coacervate-like nanosystem. We envisage that this technique can be widely utilized in the nanomedicine field to bypass the special requirement of large-scale production and elongated shelf life of nanomaterials.

Automated summary

Despite advancements in nanomedicine research, there are limited nanoformulations available on the market and few have been translated to clinics. This study introduces an instant drug formulation method using a coacervate-like nanosystem, which enhances intracellular drug delivery. The results demonstrate the feasibility of this technique and its potential application in the nanomedicine field to overcome the challenges of large-scale production and long-term storage of nanomaterials.