Photothermal Nanomaterial-Mediated Photoporation

Article Chemistry, Multidisciplinary

Delivery of macromolecules in unstimulated T cells by photoporation with polydopamine nanoparticles

Dominika Berdecka et al.

Summary: Ex vivo modification of T cells is essential for T cell therapies. Photoporation using small photothermal nanoparticles (PDNPs) is a promising method to deliver large molecules into quiescent T cells.

JOURNAL OF CONTROLLED RELEASE (2023)

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Article Biochemistry & Molecular Biology

Response Surface Methodology to Efficiently Optimize Intracellular Delivery by Photoporation

Ilia Goemaere et al.

Summary: Photoporation is a gentle and efficient method for transfecting cells, and response surface methodology (RSM) proves to be a valuable approach for optimizing the photoporation procedure. By varying PDNP size, concentration, and laser fluence, the RSM designs successfully identified an optimal delivery yield and revealed a strong dependence on PDNP size.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)

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Article Biochemistry & Molecular Biology

Transient nuclear lamin A/C accretion aids in recovery from vapor nanobubble-induced permeabilisation of the plasma membrane

Gaelle Houthaeve et al.

Summary: The study investigates the effects of VNB photoporation on cell homeostasis, finding that A-type lamins are upregulated after photoporation and contribute to the recovery of the nucleus. Selective depletion of A-type lamins reduces cell viability, while stimulation of LMNA transcription increases the survival rate of successfully transfected cells.

CELLULAR AND MOLECULAR LIFE SCIENCES (2022)

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Article Nanoscience & Nanotechnology

Laser-induced nanobubbles safely ablate vitreous opacities in vivo

Felix Sauvage et al.

Summary: This paper introduces a new method for treating eye floaters, involving the generation of vapour nanobubbles by exposing gold nanoparticles or indocyanine green to low-energy laser pulses to disrupt collagen aggregates in the eyes. This approach may provide a safe and efficient treatment for millions of patients suffering from debilitating vitreous opacities and opens up the possibility of highly safe use of pulsed lasers in the posterior segment of the eye.

NATURE NANOTECHNOLOGY (2022)

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Article Multidisciplinary Sciences

Light triggered nanoscale biolistics for efficient intracellular delivery of functional macromolecules in mammalian cells

Juan C. Fraire et al.

Summary: Biolistic delivery with light-triggered self-assembled nanobombs can efficiently transfect mammalian cells by propelling smaller nanoprojectiles through the cell membrane using vapor bubble formation. It outperforms electroporation in terms of transfection yield.

NATURE COMMUNICATIONS (2022)

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Article Chemistry, Multidisciplinary

ICG-mediated photodisruption of the inner limiting membrane enhances retinal drug delivery

Karen Peynshaert et al.

Summary: This study presents a light-based approach to overcome the barrier of the inner limiting membrane (ILM) in retinal therapies, improving their efficacy. Evidence is provided through the use of pulsed laser irradiation and the formation of vapor nanobubbles.

JOURNAL OF CONTROLLED RELEASE (2022)

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Article Pharmacology & Pharmacy

Photodisruption of the Inner Limiting Membrane: Exploring ICG Loaded Nanoparticles as Photosensitizers

Kaat De Clerck et al.

Summary: Vapor nanobubbles created by photosensitizer-induced perforation of the inner limiting membrane can overcome the barrier for efficient drug delivery to the retina. Indocyanine green loaded nanoparticles, particularly ICG liposomes, show high ICG encapsulation and VNB generation capacity, resulting in subtle disruption effects on the ILM.

PHARMACEUTICS (2022)

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Article Nanoscience & Nanotechnology

Photothermal nanofibres enable safe engineering of therapeutic cells

Ranhua Xiong et al.

Summary: Photothermal nanofibres embedded with iron oxide nanoparticles can deliver effector macromolecules to different types of cells without causing cellular toxicity or diminishing therapeutic potency, showing promise for safe and efficient production of engineered cells for therapeutic applications.

NATURE NANOTECHNOLOGY (2021)

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Article Chemistry, Multidisciplinary

Photoporation with Biodegradable Polydopamine Nanosensitizers Enables Safe and Efficient Delivery of mRNA in Human T Cells

Aranit Harizaj et al.

Summary: The study investigated the possibility of using biocompatible polymeric nanoparticles to form laser-induced vapor nanobubbles as an efficient means of photoporation. Compared to electroporation, photoporation sensitized by polydopamine nanoparticles showed better results for transfecting T cells.

ADVANCED FUNCTIONAL MATERIALS (2021)

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Article Nanoscience & Nanotechnology

Cellular binding, uptake and biotransformation of silver nanoparticles in human T lymphocytes

Anzhela Malysheva et al.

Summary: The study demonstrates the presence of silver nanoparticles in nanoparticulate form after being taken up by human lymphocytes. Biotransformation of AgNPs is primarily dominated by sulfidation, serving as a detoxification pathway for cells. Toxicity of AgNPs is mainly driven by internalized silver, with differences in toxicity between nanoparticles of different coatings and sizes being minimized when expressed as intracellular mass of silver per cell.

NATURE NANOTECHNOLOGY (2021)

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Article Chemistry, Multidisciplinary

Bubble‐Forming Films: Bubble Forming Films for Spatial Selective Cell Killing (Adv. Mater. 27/2021)

Dawei Hua et al.

ADVANCED MATERIALS (2021)

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Review Biotechnology & Applied Microbiology

Non-viral transfection technologies for next-generation therapeutic T cell engineering

Laurens Raes et al.

Summary: Genetically engineered T cells have shown promising potential in cancer treatment, with a focus on CAR T cell therapies. The next generation of CAR T cell therapies will require more complex engineering strategies and may involve revolutionary gene editing approaches. Non-viral transfection technologies are gaining interest as alternatives to viral vectors, with the potential to enhance the manufacturing process and improve safety considerations.

BIOTECHNOLOGY ADVANCES (2021)

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Review Chemistry, Physical

Vapor nanobubble-mediated photoporation constitutes a versatile intracellular delivery technology Jana Ramon, Ranhua Xiong, Stefaan C. De Smedt, Koen Raemdonck and Kevin Braeckmans

Jana Ramon et al.

Summary: Vapor nanobubble-mediated photoporation is a promising physical intracellular delivery technology that creates transient membrane pores through the collapse of vapor nanobubbles, allowing exogenous molecules to enter cells. It offers high-throughput and spatially defined delivery even at single-cell resolution, making it useful for targeting specific cells in heterogeneous samples. Additionally, this strategy can not only permeabilize the outer cell membrane but also disrupt endosomal and nuclear membranes.

CURRENT OPINION IN COLLOID & INTERFACE SCIENCE (2021)

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Article Medicine, Research & Experimental

Cas9 RNP transfection by vapor nanobubble photoporation for ex vivo cell engineering

Laurens Raes et al.

Summary: The study successfully demonstrated Cas9 RNP transfection in various cell types using VNB photoporation, including refractive cells and achieving high gene knock-out levels. Gene knock-out according to user-defined spatial patterns was shown in stem cells and T cells. VNB photoporation is a scalable and versatile intracellular delivery method with great promise for CRISPR-Cas9-mediated ex vivo engineering of cell therapy products.

MOLECULAR THERAPY-NUCLEIC ACIDS (2021)

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Article Chemistry, Multidisciplinary

Black phosphorus mediated photoporation: a broad absorption nanoplatform for intracellular delivery of macromolecules

Jielin Wang et al.

Summary: The study explores black phosphorus quantum dots (BPQDs) as alternative sensitizing nanoparticles with a broad and uniform absorption spectrum for photoporation. BPQD sensitized photoporation efficiently delivers siRNA and mRNA into cells with high cell viability under different laser wavelengths. Furthermore, the delivery efficiency remains relatively high even with thick phantom tissue in the optical path.

NANOSCALE (2021)

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Article Chemistry, Multidisciplinary

The fragmentation mechanism of gold nanoparticles in water under femtosecond laser irradiation

Gabriele Bongiovanni et al.

Summary: This study used in situ electron microscopy to directly observe the femtosecond laser-induced fragmentation of gold nanoparticles in water, revealing that the process occurs through ejection of individual progeny particles. The fragmentation mechanism involves Coulomb fission and solution-mediated etching and growth processes, creating complex fragmentation patterns that rapidly fluctuate under prolonged irradiation. This study highlights the complexity of the interaction of plasmonic nanoparticles with ultrafast laser pulses and underlines the need for in situ observations to unravel the mechanisms of related phenomena.

NANOSCALE ADVANCES (2021)

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Article Chemistry, Physical

Carbon quantum dots as a dual platform for the inhibition and light-based destruction of collagen fibers: implications for the treatment of eye floaters

Alexandre Barras et al.

Summary: Vitreous opacities caused by clumped collagen fibers can be treated with light-responsive nanostructures, which interfere with collagen aggregation to improve vision.

NANOSCALE HORIZONS (2021)

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Article Materials Science, Biomaterials

Cytosolic delivery of gadolinium via photoporation enables improved in vivo magnetic resonance imaging of cancer cells

Aranit Harizaj et al.

Summary: Longitudinal in vivo monitoring of transplanted cells is crucial for cancer research and treatment assessment. MRI offers high spatial resolution and soft-tissue contrast, but suffers from low sensitivity, requiring high concentrations of contrast agents. Photoporation provides an attractive alternative for efficient delivery of contrast agents into the cytosol, avoiding the quenching effect and improving MR signal intensity.

BIOMATERIALS SCIENCE (2021)

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Article Chemistry, Multidisciplinary

Nanoparticle-sensitized photoporation enables inflammasome activation studies in targeted single cells†

Aranit Harizaj et al.

Summary: Inflammasomes are complex protein complexes that guard against cellular stress and infections, and nanoparticle-sensitized photoporation has been shown to effectively deliver pathogenic virulence factors into primary macrophages for inflammasome activation studies.

NANOSCALE (2021)

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Review Chemistry, Multidisciplinary

Stimuli-responsive nanobubbles for biomedical applications

Ranhua Xiong et al.

Summary: This review discusses the recent advances in stimuli-responsive nanobubbles for biomedical applications, highlighting their properties, synthesis methods, and potential advantages and limitations.

CHEMICAL SOCIETY REVIEWS (2021)

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Article Biochemistry & Molecular Biology

Surface Functionalization with Polyethylene Glycol and Polyethyleneimine Improves the Performance of Graphene-Based Materials for Safe and Efficient Intracellular Delivery by Laser-Induced Photoporation

Jing Liu et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

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Article Chemistry, Physical

Long-term live-cell microscopy with labeled nanobodies delivered by laser-induced photoporation

Jing Liu et al.

NANO RESEARCH (2020)

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Article Nanoscience & Nanotechnology

Intracellular Delivery of mRNA in Adherent and Suspension Cells by Vapor Nanobubble Photoporation

Laurens Raes et al.

NANO-MICRO LETTERS (2020)

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Article Biochemical Research Methods

Nanobody click chemistry for convenient site-specific fluorescent labelling, single step immunocytochemistry and delivery into living cells by photoporation and live cell imaging

Tim Hebbrecht et al.

NEW BIOTECHNOLOGY (2020)

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Article Pharmacology & Pharmacy

Exploring Light-Sensitive Nanocarriers for Simultaneous Triggered Antibiotic Release and Disruption of Biofilms Upon Generation of Laser-Induced Vapor Nanobubbles

Eline Teirlinck et al.

PHARMACEUTICS (2019)

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Article Chemistry, Multidisciplinary

Photoablation of Human Vitreous Opacities by Light-Induced Vapor Nanobubbles

Felix Sauvage et al.

ACS NANO (2019)

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Article Biochemistry & Molecular Biology

Delivery of Mixed-Lineage Kinase Domain-Like Protein by Vapor Nanobubble Photoporation Induces Necroptotic-Like Cell Death in Tumor Cells

Lien Van Hoecke et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019)

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Article Crystallography

Gold Nanoparticle-Mediated Photoporation Enables Delivery of Macromolecules over a Wide Range of Molecular Weights in Human CD4+T Cells

Laurens Raes et al.

CRYSTALS (2019)

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Article Chemistry, Multidisciplinary

Targeted Perturbation of Nuclear Envelope Integrity with Vapor Nanobubble-Mediated Photoporation

Gaelle Houthaeve et al.

ACS NANO (2018)

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Review Pharmacology & Pharmacy

Advanced physical techniques for gene delivery based on membrane perforation

Xiaofan Du et al.

DRUG DELIVERY (2018)

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Article Chemistry, Multidisciplinary

Degradation Chemistry and Stabilization of Exfoliated Few-Layer Black Phosphorus in Water

Taiming Zhang et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2018)

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Article Neurosciences

Selective Labeling of Individual Neurons in Dense Cultured Networks With Nanoparticle-Enhanced Photoporation

Ranhua Xiong et al.

FRONTIERS IN CELLULAR NEUROSCIENCE (2018)

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Review Chemistry, Multidisciplinary

Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts

Martin P. Stewart et al.

CHEMICAL REVIEWS (2018)

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Article Optics

Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy

Jing Liu et al.

LIGHT-SCIENCE & APPLICATIONS (2018)

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Article Chemistry, Multidisciplinary

In Vivo Laser-Mediated Retinal Ganglion Cell Optoporation Using Kv1.1 Conjugated Gold Nanoparticles

Ariel M. Wilson et al.

NANO LETTERS (2018)

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Article Multidisciplinary Sciences

Laser-induced vapour nanobubbles improve drug diffusion and efficiency in bacterial biofilms

Eline Teirlinck et al.

NATURE COMMUNICATIONS (2018)

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Article Chemistry, Multidisciplinary

Comparing photoporation and nucleofection for delivery of small interfering RNA to cytotoxic T cells

Laura Wayteck et al.

JOURNAL OF CONTROLLED RELEASE (2017)

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Article Chemistry, Multidisciplinary

Fast spatial-selective delivery into live cells

Ranhua Xiong et al.

JOURNAL OF CONTROLLED RELEASE (2017)

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Article Chemistry, Multidisciplinary

Mimicking Melanosomes: Polydopamine Nanoparticles as Artificial Microparasols

Yuran Huang et al.

ACS CENTRAL SCIENCE (2017)

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Article Chemistry, Multidisciplinary

Cytosolic Delivery of Nanolabels Prevents Their Asymmetric Inheritance and Enables Extended Quantitative in Vivo Cell Imaging

Ranhua Xiong et al.

NANO LETTERS (2016)

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Review Multidisciplinary Sciences

In vitro and ex vivo strategies for intracellular delivery

Martin P. Stewart et al.

NATURE (2016)

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Article Nanoscience & Nanotechnology

Intraoperative diagnostics and elimination of residual microtumours with plasmonic nanobubbles

Ekaterina Y. Lukianova-Hleb et al.

NATURE NANOTECHNOLOGY (2016)

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Review Physics, Multidisciplinary

Laser-assisted photoporation: fundamentals, technological advances and applications

Ranhua Xiong et al.

ADVANCES IN PHYSICS-X (2016)

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Review Chemistry, Multidisciplinary

Carbon Nanomaterials for Biological Imaging and Nanomedicinal Therapy

Guosong Hong et al.

CHEMICAL REVIEWS (2015)

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Article Chemistry, Multidisciplinary

Efficient Intracellular Delivery of Molecules with High Cell Viability Using Nanosecond-Pulsed Laser-Activated Carbon Nanoparticles

Aritra Sengupta et al.

ACS NANO (2014)

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Article Chemistry, Multidisciplinary

Comparison of Gold Nanoparticle Mediated Photoporation: Vapor Nanobubbles Outperform Direct Heating for Delivering Macromolecules in Live Cells

Ranhua Xiong et al.

ACS NANO (2014)

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Review Chemistry, Multidisciplinary

Polydopamine and Its Derivative Materials: Synthesis and Promising Applications in Energy, Environmental, and Biomedical Fields

Yanlan Liu et al.

CHEMICAL REVIEWS (2014)

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Article Nanoscience & Nanotechnology

From Thermo- to Plasma-Mediated Ultrafast Laser-Induced Plasmonic Nanobubbles

Remi Lachaine et al.

ACS PHOTONICS (2014)

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Review Chemistry, Multidisciplinary

Assessing nanoparticle toxicity in cell-based assays: influence of cell culture parameters and optimized models for bridging the in vitro-in vivo gap

Freya Joris et al.

CHEMICAL SOCIETY REVIEWS (2013)

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Article Chemistry, Multidisciplinary

Cytotoxic Effects of Gold Nanoparticles: A Multiparametric Study

Stefaan J. Soenen et al.

ACS NANO (2012)

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Article Engineering, Biomedical

Cell-specific transmembrane injection of molecular cargo with gold nanoparticle-generated transient plasmonic nanobubbles

Ekaterina Y. Lukianova-Hleb et al.

BIOMATERIALS (2012)

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Article Physics, Multidisciplinary

Thermodynamics of nanosecond nanobubble formation at laser-excited metal nanoparticles

A. Siems et al.

NEW JOURNAL OF PHYSICS (2011)

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Review Optics

Optical excitation and detection of vapor bubbles around plasmonic nanoparticles

Dmitri Lapotko

OPTICS EXPRESS (2009)

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Photothermal Nanomaterial-Mediated Photoporation

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