Journal
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Volume 36, Issue 1, Pages 179-190Publisher
KARGER
DOI: 10.1159/000374062
Keywords
Ischemia/Reperfusion injury; miR-31; PKCe; NF kappa B
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Funding
- National Natural Science Foundation of China [81200149]
- Medicine/Engineering Cross Fund of Shanghai Jiaotong University [YG2013MS35]
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Background: Various miRNAs have been shown to participate in cardiac ischemia/reperfusion injury (I/R). miR-31 was identified as the most strikingly upregulated miRNA after acute myocardial infarction; therefore, the underlying role and mechanism of miR-31 in cardiac I/R was investigated. Methods: miR-31 expression was detected after cardiac I/R in mice. The cardioprotective effect of miR-31 downregulation was assessed in vitro and in vivo. The functional target gene and its downstream molecule were determined. Results: miR-31 expression increased after I/R. miR-31 downregulation increased cell viability and SOD activity and decreased LDH activity and MDA content in vitro. Additionally, miR-31 downregulation alleviated myocardial infarct size in vivo. PKCe was identified as the functional target gene of miR-31, and NF kappa B was identified as its downstream molecule that was involved in the miR-31-mediated cardioprotective effect. Conclusion: miR-31 expression increased throughout the cardiac I/R process, and miR-31 downregulation induced a cardioprotective effect via a miR-31/ PKC epsilon/NF kappa B-dependent pathway. Copyright (C) 2015 S. Karger AG, Basel
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