Journal
COSMETICS
Volume 9, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/cosmetics9050106
Keywords
cosmeceuticals; protease; Arthrospira platensis; aqueous two-phase system; hydrogel
Categories
Funding
- European Union [778263]
- European Union (European Social Fund (ESF)) through the Operational Programme Human Resources Development, Education and Lifelong Learning [MIS-5000432]
- Marie Curie Actions (MSCA) [778263] Funding Source: Marie Curie Actions (MSCA)
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Microalgae are a valuable source of enzymes and active ingredients. The proteolytic activity of Arthrospira platensis lysate was assessed and characterized, and a rapid and easy purification protocol was developed. The proteolytic activity of A. platensis lysate was used to develop a hydrogel formulation, which showed improved operational stability.
Microalgae are a valuable source of enzymes and active ingredients due to their biochemical variability, health-beneficial properties, lower production cost and viability on an industrial scale. In addition, the growing demand for sustainable products constitutes microalgae as a viable resource for the development of a range of innovative products. In the present work, the proteolytic activity of Arthrospira platensis lysate was assessed and characterized using kinetics analysis and zymography employing different substrates, pH values and divalent ions. The results suggest the presence of two main proteolytic enzymes in the lysate. In addition, a rapid and easy purification protocol of the proteolytic activity, under mild conditions, was developed using a polyethyleneglycol (PEG)/phosphate aqueous two-phase system (ATPS). The proteolytic activity of A. platensis lysate was used to develop a hydrogel formulation as an enzyme-based cosmeceutical, with potential application as a topical exfoliating agent. The incorporation of the A. platensis extract in the developed hydrogel formulation significantly improved its operational stability over time, which is a significant advantage in enzyme-based product development.
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