Journal
BIOMATERIALS RESEARCH
Volume 27, Issue 1, Pages -Publisher
SPRINGERNATURE
DOI: 10.1186/s40824-023-00349-y
Keywords
Decellularized cartilage extracellular matrix; Low-temperature deposition manufacturing; Tissue engineering; Articular cartilage regeneration
Ask authors/readers for more resources
In this study, a hierarchical porous ECM scaffold with GDF-5 was successfully fabricated using ECM as a bioink in LDM 3D printing. The 7% ECM scaffold showed the best biocompatibility and the addition of GDF-5 significantly improved BMSC migration and chondrogenic differentiation. In vivo results demonstrated that the ECM/GDF-5 scaffold enhanced in situ cartilage repair.
BackgroundIn recent years, there has been significant research progress on in situ articular cartilage (AC) tissue engineering with endogenous stem cells, which uses biological materials or bioactive factors to improve the regeneration microenvironment and recruit more endogenous stem cells from the joint cavity to the defect area to promote cartilage regeneration.MethodIn this study, we used ECM alone as a bioink in low-temperature deposition manufacturing (LDM) 3D printing and then successfully fabricated a hierarchical porous ECM scaffold incorporating GDF-5.ResultsComparative in vitro experiments showed that the 7% ECM scaffolds had the best biocompatibility. After the addition of GDF-5 protein, the ECM scaffolds significantly improved bone marrow mesenchymal stem cell (BMSC) migration and chondrogenic differentiation. Most importantly, the in vivo results showed that the ECM/GDF-5 scaffold significantly enhanced in situ cartilage repair.ConclusionIn conclusion, this study reports the construction of a new scaffold based on the concept of in situ regeneration, and we believe that our findings will provide a new treatment strategy for AC defect repair.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available