The Fate of Phosphorus in Experimental Burials: Chemical and Ultramicroscopic Characterization and Environmental Control of Its Persistency

The permanence of a buried body in soil always induces the formation of a decomposition island that can serve as a significant recording location to understand how the persistence of a clandestine grave affects soil. This study aims to analyze the elemental exchange from buried bodies to soil, with a focus on phosphorus content, and to determine the effects of environmental factors on its persistency. The experiment was carried out using eleven swine carcasses buried in an open site (northern Italy). The analyses were performed using the Olsen P method, which allowed for a recognition of the trend of the amount of phosphorus over time, due to the decomposition of phospholipids, followed by the transfer of the element from bone to soil. Additionally, microanalyses performed using a scanning electron microscope (SEM-EDS) on two different soil sample specimens (i.e., dust and plug) allowed for the identification of numerous phosphatic features (i.e., coatings, infillings, impregnations, and organo-mineral associations), which are the result of the interaction between soil and body fluids and can thus be used as indicators of the former presence of decomposing body (even in its absence). The ultramicroscopic analysis also shows increasing and decreasing amounts of P2O5 over time in the soil, which could be related to environmental conditions (i.e., soil moisture), due to the leaching of phosphorus induced by the percolation of natural rainwater. The study underlines the potential use of these methods to evaluate the possibility of a cadaver-soil linkage and of assessing the burial in the soil for a variable period. Moreover, the study may aid in analyzing the dynamics of phosphorus migration from buried bodies to soil during and after the decomposition process.

Automated summary

This study aimed to analyze the exchange of elements from buried bodies to soil, focusing on phosphorus content, and to determine the effects of environmental factors on its persistence. The experiment involved burying eleven swine carcasses in an open site, with analyses revealing the decomposition of phospholipids and transfer of phosphorus from bone to soil. Microanalyses of soil samples identified phosphatic features resulting from the interaction between soil and body fluids, and ultramicroscopic analysis showed fluctuations in P2O5 in the soil over time. These methods have potential for evaluating the cadaver-soil linkage, burial duration, and phosphorus migration during and after decomposition.