In vitro antitumor immune response induced by dendritic cells transduced with human livin α recombinant adenovirus

Transduction with recombinant, replication-defective adenoviral (rAd) vectors encoding a transgene is an efficient method for gene transfer into human dendritic cells (DCs). Livin is a good candidate for cancer immunotherapy since it is overexpressed in most common human cancers, poorly expressed in most normal adult tissues. Two splicing variants of livin, designated livin alpha and livin beta, have been identified. In this study, we used human livin a recombinant adenovirus (rAd-hlivin alpha) to transduced DCs. We found that DCs transduced with rAd-hlivin alpha (rAd-hlivin alpha DCs) could effectively induce human livin alpha specific cytotoxic T lymphocytes (CTL) in vitro against various tumor cell lines. (C) 2015 Elsevier Inc. All rights reserved.