Journal
JOURNAL OF STRUCTURAL BIOLOGY
Volume 194, Issue 3, Pages 423-433Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2016.04.007
Keywords
Electron microscopy density map; Single particle analysis; Denoising; Macromolecular complexes; Structure; Gaussian functions
Funding
- CNRS (France)
- CSIC (Spain) [Projet International de Cooperation Scientifique - PICS]
- French National Research Agency ANR [ANR-11-BSV8-010-04]
- Spanish Ministry of Economy and Competitiveness [AIC-A-2011-0638, BIO2013-44647-R]
- Comunidad de Madrid [CAM S2010/BMD-2305]
- European Social Fund
- Ministerio de Educacion y Ciencia (Ramon y Cajal fellowship)
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Cryo-electron microscopy (cryo-EM) of frozen-hydrated preparations of isolated macromolecular complexes is the method of choice to obtain the structure of complexes that cannot be easily studied by other experimental methods due to their flexibility or large size. An increasing number of macromolecular structures are currently being obtained at subnanometer resolution but the interpretation of structural details in such EM-derived maps is often difficult because of noise at these high-frequency signal components that reduces their contrast. In this paper, we show that the method for EM density-map approximation using Gaussian functions can be used for denoising of single-particle EM maps of high (typically subnanometer) resolution. We show its denoising performance using simulated and experimental EM density maps of several complexes. (C) 2016 Published by Elsevier Inc.
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