3.8 Review

Non-invasive evaluation of embryo quality for the selection of transferable embryos in human in vitro fertilization-embryo transfer

Related references

Note: Only part of the references are listed.
Article Obstetrics & Gynecology

Machine learning for prediction of euploidy in human embryos: in search of the best-performing model and predictive features

Stefanie De Gheselle et al.

Summary: This study assessed various machine learning models and features to predict euploidy in human embryos, and found that the random forest classifier model performed the best. The study also showed that morphokinetic features had the highest relative predictive weight.

FERTILITY AND STERILITY (2022)

Article Obstetrics & Gynecology

A double-blind randomized controlled trial investigating a time-lapse algorithm for selecting Day 5 blastocysts for transfer

Aisling Ahlstrom et al.

Summary: The use of a commercially available time-lapse algorithm for Day 5 blastocyst selection did not improve ongoing pregnancy rates compared with morphology alone.

HUMAN REPRODUCTION (2022)

Article Obstetrics & Gynecology

Metabolic state of human blastocysts measured by fluorescence lifetime imaging microscopy

Marta Venturas et al.

Summary: Fluorescence lifetime imaging microscopy (FLIM) can detect variations in metabolic profiles between discarded human blastocysts. The study revealed extensive metabolic variations associated with blastocyst development, as well as metabolic heterogeneity within individual blastocysts, suggesting FLIM as a promising approach for evaluating embryo viability.

HUMAN REPRODUCTION (2022)

Article Genetics & Heredity

GDF9 concentration in embryo culture medium is linked to human embryo quality and viability

Jingyu Li et al.

Summary: The study evaluated the association between GDF9 concentration in human embryo culture medium and embryo quality and viability. Results showed that GDF9 concentration was linked to embryo quality and viability, suggesting it could serve as a non-invasive biomarker for embryo selection.

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS (2022)

Article Multidisciplinary Sciences

Robust and generalizable embryo selection based on artificial intelligence and time-lapse image sequences

Jorgen Berntsen et al.

Summary: Assessing and selecting the most viable embryos for transfer is crucial in IVF, and the use of AI and deep learning has improved and automated this process. This study investigates a deep learning-based embryo selection model and its performance across different patient ages and clinical conditions, showing its correlation with traditional morphokinetic parameters. The model was trained and evaluated using a large dataset from 18 IVF centers, demonstrating its effectiveness and generalization to new clinics.

PLOS ONE (2022)

Article Endocrinology & Metabolism

Improved Non-Invasive Preimplantation Genetic Testing for Beta-Thalassemia Using Spent Embryo Culture Medium Containing Blastocoelic Fluid

Zhanhui Ou et al.

Summary: This study compared the detection rates of beta-thalassemia using spent culture medium and spent culture medium containing blastocoelic fluid. The results showed that using spent culture medium containing blastocoelic fluid had a higher detection rate, and the DNA concentration in the spent culture medium was associated with fragmentation.

FRONTIERS IN ENDOCRINOLOGY (2022)

Article Obstetrics & Gynecology

Noninvasive embryo evaluation and selection by time-lapse monitoring vs. conventional morphologic assessment in women undergoing in vitro fertilizati on/intracytoplasmic sperm injection: a single-center randomized controlled study

Qingxia Meng et al.

Summary: This prospective randomized controlled trial aimed to compare the effectiveness of time-lapse monitoring (TLM) and conventional morphological assessment (CMA) for embryo selection. The results showed that the clinical pregnancy rate (CPR) and live birth rate (LBR) were significantly lower in the TLM group compared to the CMA group. However, there was no significant difference in CPR between the TLM and CMA groups for fresh embryo transfer or FET.

FERTILITY AND STERILITY (2022)

Article Biochemistry & Molecular Biology

LC-MS Analysis Revealed the Significantly Different Metabolic Profiles in Spent Culture Media of Human Embryos with Distinct Morphology, Karyotype and Implantation Outcomes

Chupalav Eldarov et al.

Summary: In this study, metabolomic analysis of spent embryo culture media (SECM) revealed differences in metabolomic profiles between human embryos with different morphology and karyotype. Additionally, significant changes in metabolomic profiles were observed in SECM from embryos with successful implantation outcomes. Separate analysis of SECM samples on different days showed distinct molecular signatures in embryos with successful outcomes.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

Article Obstetrics & Gynecology

Comparison of pregnancy outcomes using a time-lapse monitoring system for embryo incubation versus a conventional incubator in in vitro fertilization: An age-stratification analysis

Pattraporn Chera-aree et al.

Summary: This study found that embryos cultured in a time-lapse monitoring system had significantly better pregnancy outcomes in in vitro fertilization compared to those cultured in a conventional incubator, particularly in advanced age women.

CLINICAL AND EXPERIMENTAL REPRODUCTIVE MEDICINE-CERM (2021)

Article Obstetrics & Gynecology

An artificial intelligence model based on the proteomic profile of euploid embryos and blastocyst morphology: a preliminary study

Lorena Bori et al.

Summary: This study aimed to develop an artificial intelligence model based on artificial neural networks to predict the likelihood of achieving a live birth using the proteomic profile of spent culture media and blastocyst morphology. The results showed that the artificial intelligence model utilizing information extracted from blastocyst image analysis and specific protein concentrations was able to accurately predict live birth.

REPRODUCTIVE BIOMEDICINE ONLINE (2021)

Article Genetics & Heredity

Early cleaving embryos result in blastocysts with increased aspartate and glucose consumption, which exhibit different metabolic gene expression that persists in placental and fetal tissues

Y. S. L. Lee et al.

Summary: This study developed a targeted enzymatic assay to quantify aspartate uptake in single blastocysts. Results showed that 'fast' blastocysts had significantly higher rates of aspartate and glucose consumption. Gene expression analysis revealed that genes involved in aspartate and glucose metabolism were increased in 'fast' blastocysts, with differences maintained in placental and fetal liver tissue.

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS (2021)

Article Endocrinology & Metabolism

An artificial intelligence model (euploid prediction algorithm) can predict embryo ploidy status based on time-lapse data

Bo Huang et al.

Summary: This study investigates the relationship between time-lapse technology and embryo ploidy status, proposing an AI model named EPA to predict embryo ploidy based on TLT data. The model showed good prediction accuracy on testing datasets, opening up possibilities for non-invasive embryo selection in in vitro fertilization.

REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY (2021)

Article Multidisciplinary Sciences

Cell-free DNA in spent culture medium effectively reflects the chromosomal status of embryos following culturing beyond implantation compared to trophectoderm biopsy

Akihiro Shitara et al.

Summary: The study found that niPGT-A may be more accurate than PGT-A in terms of ploidy diagnostic accuracy in outgrowths.

PLOS ONE (2021)

Article Endocrinology & Metabolism

Validation of preimplantation genetic tests for aneuploidy (PGT-A) with DNA from spent culture media (SCM): concordance assessment and implication

Baoli Yin et al.

Summary: Using spent culture medium (SCM) as a source of DNA for preimplantation genetic testing aneuploidy (PGT-A) has been widely discussed. However, our study found that DNA in SCM samples had lower quality and quantity compared to TE samples, leading to lower interpretability and full chromosomal concordance rates. TE still remains a more accurate and reliable high-throughput methodology for PGT-A.

REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY (2021)

Article Obstetrics & Gynecology

Comparison of single euploid blastocyst transfer cycle outcome derived from embryos with normal or abnormal cleavage patterns

Irem Yarali Ozbek et al.

Summary: The study aimed to assess abnormal cleavage in biopsied blastocysts, compare euploidy rates of blastocysts with abnormal and normal cleavage, and analyze single euploid blastocyst transfer (SEBT) outcomes. Results showed blastocysts with abnormal cleavage had higher euploidy rates compared to those with normal cleavage, but lower live birth rates after SEBT. Abnormal cleavage pattern was the only independent predictor of live birth rate, suggesting blastocysts with abnormal cleavage should have lower priority for transfer.

REPRODUCTIVE BIOMEDICINE ONLINE (2021)

Article Obstetrics & Gynecology

Noninvasive preimplantation genetic testing for aneuploidy exhibits high rates of deoxyribonucleic acid amplification failure and poor correlation with results obtained using trophectoderm biopsy

Brent M. Hanson et al.

Summary: The study validated a commercially available noninvasive preimplantation genetic testing for aneuploidy (niPGT-A) assay, investigating DNA amplification failure rates, factors affecting amplification failure, and discordant results between niPGT-A and traditional preimplantation genetic testing for aneuploidy. The findings revealed high rates of DNA amplification failure with niPGT-A, limiting its clinical applicability in its current form. Whole-chromosome discordance was noted in 40.4% of cases between niPGT-A and TE biopsy results.

FERTILITY AND STERILITY (2021)

Article Pharmacology & Pharmacy

Digital PCR Detection of mtDNA/gDNA Ratio in Embryo Culture Medium for Prediction of Embryo Development Potential

Qing Zhang et al.

Summary: This study aimed to define the predictive value of the mtDNA/gDNA ratio for embryonic developmental potential, showing a correlation between the ratio and embryo development in culture medium. Results demonstrated that the ratio decreased with lower embryo ratings, with differences in trends between formed and unformed blastocysts.

PHARMACOGENOMICS & PERSONALIZED MEDICINE (2021)

Article Veterinary Sciences

Does miRNA Expression in the Spent Media Change During Early Embryo Development?

Paul Del Rio et al.

Summary: Distinct miRNA populations have been detected in the spent media of in-vitro culture systems throughout the pre-implantation period, with a total of 111 miRNAs identified. These miRNAs may impact early embryo development by regulating genes involved in cellular processes, metabolic processes, and biological regulation.

FRONTIERS IN VETERINARY SCIENCE (2021)

Article Obstetrics & Gynecology

Pregnancy prediction performance of an annotation-free embryo scoring system on the basis of deep learning after single vitrified-warmed blastocyst transfer: a single-center large cohort retrospective study

Satoshi Ueno et al.

Summary: The study found that the annotation-free embryo scoring system iDAScore outperformed other systems relying on annotation or morphology scores in predicting pregnancy outcomes after single vitrified blastocyst transfer (SVBT), especially in young patients.

FERTILITY AND STERILITY (2021)

Article Genetics & Heredity

Preimplantation Genetic Testing for Monogenic Conditions: Is Cell-Free DNA Testing the Next Step?

Alice Rogers et al.

Summary: Preimplantation genetic testing offers an important reproductive option for couples seeking to improve IVF success rates and reduce genetic disease risks. Non-invasive PGT may provide an alternate mode of embryonic analysis, with potential for high-risk couples in situations where traditional testing is not possible.

MOLECULAR DIAGNOSIS & THERAPY (2021)

Article Endocrinology & Metabolism

Comparing prediction of ongoing pregnancy and live birth outcomes in patients with advanced and younger maternal age patients using KIDScore™ day 5: a large-cohort retrospective study with single vitrified-warmed blastocyst transfer

Keiichi Kato et al.

Summary: The study showed that the KIDScore model performed well in predicting pregnancy and live birth outcomes in advanced age patients. The older the maternal age, the higher the accuracy of the model's predictions.

REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY (2021)

Article Multidisciplinary Sciences

Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis

Jin Huang et al.

Summary: NICS technology may offer a solution to the problems in the prevailing PGT-A method, but its efficacy has been limited. A new study reports the full protocol of NICS, including culture medium sampling, whole genome amplification, and data analysis methods. Cryopreservation times in different embryo laboratories influence the choice of embryo culture medium collection methods.

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS (2021)

Article Medicine, General & Internal

Change in the Strategy of Embryo Selection with Time-Lapse System Implementation-Impact on Clinical Pregnancy Rates

Lisa Boucret et al.

Summary: The study evaluated how TLS and the KIDScore algorithm changed embryo selection practices and improved clinical pregnancy rates compared to conventional morphological evaluation. Findings showed that the TLS group in 2020 had significantly higher CPR compared to the conventional group in 2019, indicating that TLS is useful in identifying embryos with the highest potential for pregnancy.

JOURNAL OF CLINICAL MEDICINE (2021)

Article Medicine, Research & Experimental

Embryonic Cell-free DNA in Spent Culture Medium: A Non-invasive Tool for Aneuploidy Screening of the Corresponding Embryos

Afrodite Sialakouma et al.

Summary: The study found good agreement between TE and SCM analysis, suggesting that niPGT-A could be a reliable alternative for chromosomal abnormalities assessment of in vitro cultured embryos.

IN VIVO (2021)

Article Andrology

A machine learning system with reinforcement capacity for predicting the fate of an ART embryo

Sandrine Giscard D'Estaing et al.

Summary: The aim of this study was to construct a machine learning system-generated score to predict the fate of ART embryos incubated in a time lapse monitoring system. The DynScore showed promising predictive power for blastocyst formation in both training and global setting data groups, with the potential for further improvement through system reinforcement. The system could be adaptable to any ART center's data, maintaining its predictive capacity despite potential events during the ART process.

SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE (2021)

Review Obstetrics & Gynecology

Non-invasive preimplantation genetic testing (niPGT): the next revolution in reproductive genetics?

Megan Leaver et al.

HUMAN REPRODUCTION UPDATE (2020)

Review Medicine, General & Internal

Time-lapse technology for embryo culture and selection

Kersti Lundin et al.

UPSALA JOURNAL OF MEDICAL SCIENCES (2020)

Article Multidisciplinary Sciences

Autophagy-mediated apoptosis eliminates aneuploid cells in a mouse model of chromosome mosaicism

Shruti Singla et al.

NATURE COMMUNICATIONS (2020)

Article Biochemistry & Molecular Biology

Noninvasive preimplantation genetic testing in assisted reproductive technology: current state and future perspectives

Jingyi Li et al.

JOURNAL OF GENETICS AND GENOMICS (2020)

Article Obstetrics & Gynecology

The Kidscore™ D5 algorithm as an additional tool to morphological assessment and PGT-A in embryo selection: a time-lapse study

Eduardo Gazzo et al.

JORNAL BRASILEIRO DE REPRODUCAO ASSISTIDA (2020)

Article Obstetrics & Gynecology

Non-invasive embryo evaluation and selection using time-lapse monitoring: Results of a randomized controlled study

Peter Kovacs et al.

EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY (2019)

Article Genetics & Heredity

Performance of Day 5 KIDScore™ morphokinetic prediction models of implantation and live birth after single blastocyst transfer

Arnaud Reignier et al.

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS (2019)

Article Obstetrics & Gynecology

20. RELATIONSHIP BETWEEN EUPLOIDY RATES AND D5 KIDSCORE™ OF BLASTOCYSTS DERIVED FROM EMBRYOSCOPE

W.Y. Yap et al.

REPRODUCTIVE BIOMEDICINE ONLINE (2019)

Article Genetics & Heredity

A comparison of morphokinetic markers predicting blastocyst formation and implantation potential from two large clinical data sets

N. Zaninovic et al.

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS (2019)

Article Health Care Sciences & Services

Deep learning enables robust assessment and selection of human blastocysts after in vitro fertilization

Pegah Khosravi et al.

NPJ DIGITAL MEDICINE (2019)

Editorial Material Obstetrics & Gynecology

Status of preimplantation genetic testing and embryo selection

Santiago Munne

REPRODUCTIVE BIOMEDICINE ONLINE (2018)

Review Obstetrics & Gynecology

Non-invasive pre-implantation genetic testing of human embryos: an emerging concept

C. Farra et al.

HUMAN REPRODUCTION (2018)

Article Obstetrics & Gynecology

Retrospective study of single vitrified-warmed blastocyst transfer cycles according to the presence of morphokinetic variables

Yong Soo Hur et al.

CLINICAL AND EXPERIMENTAL REPRODUCTIVE MEDICINE-CERM (2018)

Review Pathology

Preimplantation genetic testing: current challenges and future prospects

Anver Kuliev et al.

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS (2017)

Article Multidisciplinary Sciences

Noninvasive chromosome screening of human embryos by genome sequencing of embryo culture medium for in vitro fertilization

Juanjuan Xu et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2016)

Article Obstetrics & Gynecology

External validation of a time-lapse prediction model

Thomas Freour et al.

FERTILITY AND STERILITY (2015)

Article Obstetrics & Gynecology

New strategy for diagnosing embryo implantation potential by combining proteomics and time-lapse technologies

Francisco Dominguez et al.

FERTILITY AND STERILITY (2015)

Article Genetics & Heredity

Morphokinetic parameters using time-lapse technology and day 5 embryo quality: a prospective cohort study

Ashleigh Storr et al.

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS (2015)

Article Genetics & Heredity

A predictive model for blastocyst formation based on morphokinetic parameters in time-lapse monitoring of embryo development

Robert Milewski et al.

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS (2015)

Article Genetics & Heredity

A critical appraisal of time-lapse imaging for embryo selection: where are we and where do we need to go?

Catherine Racowsky et al.

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS (2015)

Article Obstetrics & Gynecology

Cleavage pattern predicts developmental potential of day 3 human embryos produced by IVF

S. T. Yang et al.

REPRODUCTIVE BIOMEDICINE ONLINE (2015)

Article Multidisciplinary Sciences

Prediction model for aneuploidy in early human embryo development revealed by single-cell analysis

Maria Vera-Rodriguez et al.

NATURE COMMUNICATIONS (2015)

Article Genetics & Heredity

Morphokinetic analysis of cleavage stage embryos and its relationship to aneuploidy in a retrospective time-lapse imaging study

Monika Chawla et al.

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS (2015)

Article Obstetrics & Gynecology

Increasing the probability of selecting chromosomally normal embryos by time-lapse morphokinetics analysis

Natalia Basile et al.

FERTILITY AND STERILITY (2014)

Article Obstetrics & Gynecology

The human first cell cycle: impact on implantation

Jesus Aguilar et al.

REPRODUCTIVE BIOMEDICINE ONLINE (2014)

Editorial Material Obstetrics & Gynecology

Limitations of a time-lapse blastocyst prediction model: a large multicentre outcome analysis

Kirstine Kirkegaard et al.

REPRODUCTIVE BIOMEDICINE ONLINE (2014)

Review Acoustics

Time-lapse embryo imaging for improving reproductive outcomes: systematic review and meta-analysis

L. T. Polanski et al.

ULTRASOUND IN OBSTETRICS & GYNECOLOGY (2014)

Article Genetics & Heredity

The use of morphokinetic parameters to select all embryos with full capacity to implant

Sandrine Chamayou et al.

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS (2013)

Article Obstetrics & Gynecology

Retrospective analysis of outcomes after IVF using an aneuploidy risk model derived from time-lapse imaging without PGS

Alison Campbell et al.

REPRODUCTIVE BIOMEDICINE ONLINE (2013)

Article Obstetrics & Gynecology

Selection of high-potential embryos by culture in poly(dimethylsiloxane) microwells and time-lapse imaging

Shu Hashimoto et al.

FERTILITY AND STERILITY (2012)

Article Obstetrics & Gynecology

Limited implantation success of direct-cleaved human zygotes: a time-lapse study

Irene Rubio et al.

FERTILITY AND STERILITY (2012)

Article Obstetrics & Gynecology

The impact of pronuclei morphology and dynamicity on live birth outcome after time-lapse culture

A. Azzarello et al.

HUMAN REPRODUCTION (2012)

Article Obstetrics & Gynecology

Timing of cell division in human cleavage-stage embryos is linked with blastocyst formation and quality

Maria Cruz et al.

REPRODUCTIVE BIOMEDICINE ONLINE (2012)

Article Multidisciplinary Sciences

Dynamic blastomere behaviour reflects human embryo ploidy by the four-cell stage

Shawn L. Chavez et al.

NATURE COMMUNICATIONS (2012)

Article Physiology

Time-Lapse Cleavage Rating Predicts Human Embryo Viability

D. Hlinka et al.

PHYSIOLOGICAL RESEARCH (2012)

Article Obstetrics & Gynecology

The use of morphokinetics as a predictor of embryo implantation

Marcos Meseguer et al.

HUMAN REPRODUCTION (2011)

Article Developmental Biology

Which morphological scoring system is relevant in human embryo development?

Markus Montag et al.

PLACENTA (2011)

Article Obstetrics & Gynecology

Computer-assisted embryo selection: a benefit in the evaluation of embryo quality?

Goedele Paternot et al.

REPRODUCTIVE BIOMEDICINE ONLINE (2011)

Article Obstetrics & Gynecology

Dynamic microfunnel culture enhances mouse embryo development and pregnancy rates

Y. S. Heo et al.

HUMAN REPRODUCTION (2010)

Article Biotechnology & Applied Microbiology

Non-invasive imaging of human embryos before embryonic genome activation predicts development to the blastocyst stage

Connie C. Wong et al.

NATURE BIOTECHNOLOGY (2010)

Article Obstetrics & Gynecology

Time-lapse cinematography of dynamic changes occurring during in vitro development of human embryos

Yasuyuki Mio et al.

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY (2008)

Article Developmental Biology

Metabolomics and its application for non-invasive embryo assessment in IVF

Lucy Botros et al.

MOLECULAR HUMAN REPRODUCTION (2008)

Article Obstetrics & Gynecology

Interobserver and intraobserver variation in day 3 embryo grading

Allison E. Baxter Bendus et al.

FERTILITY AND STERILITY (2006)