The absence of 25-hydroxyvitamin D3-1α-hydroxylase potentiates the suppression of EAE in mice by ultraviolet light

Ultraviolet B (UVB) light suppresses the development of multiple sclerosis (MS) in patients and experimental autoimmune encephalomyelitis (EAE) in mice. Although vitamin D-3 is produced by ultraviolet light, the suppression of EAE by narrow band UVB (NBUVB) is independent of vitamin D-3. However, it is possible that the NBUVB suppression of EAE can be further influenced by 1,25-dihydroxyvitamin D-3 (1,25-(OH)(2)D-3). We used NBUVB lamps (10 KJ/m(2)) to irradiate both wild type (WT) and 1 alpha-hydroxylase knockout mice (CYP27B1 KO) that were then induced to develop EAE. There was a complete elimination of EAE development by NBUVB in the KO mice. On the other hand, the NBUVB treatment of WT mice reduced but did not eliminate the severity or incidence of EAE. This suggests that the presence of 1,25-dihydroxyvitamin D3 actually counteracts the suppressive effect of NBUVB. In support of this concept, cytolcines (IFN-gamma, IL-10) and chemokine (CCL-5) mRNA in spinal cord were reduced in wild type or eliminated in the MO mice by the NBUVB. Cytokine mRNA levels in the spinal cord correlated with clinical scores in both WT and MO mice. (C) 2016 Elsevier Ltd. All rights reserved.