THE EFFECTS OF COPRA-MANNO-OLIGOSACCHARIDE ON BACTERIUM AGGREGATION ACTIVITY AND MICROBIOLOGICAL CHANGES IN A SIMULATED DIGESTIVE TRACT

This study aimed to present the interaction of Mannooligosaccharide (MOS) produced by optimum hydrolytic reaction of the recombinant mannanase KMAN treated defatted copra meal (DCM) to bacterial aggregation activity and to microbial changes in simulated gastrointestinal tract which were two main functions of prebiotics as a decoy receptor and a substrate for bacterial fermentation, respectively. The suitable ratio of KMAN and DCM (0.8U:1mg) achieved high DCM hydrolysate-MOS production of 2.13-2.38 g/l during 6-24 h. It enhanced Limosilactobacillus reuteri KUB-AC5 growth for 0.32-0.33 h-1 and accelerated autoaggregation activity by 95.86% and 62.89% at 37 and 42 degrees C, respectively. It decreased autoaggregation activities of Salmonella Enteritidis DMST17368 by 75.03-81.66%, and Lactobacillus johnsonii KUNN19-2 by 38.68-55.63% at 24 h. By simulated human GI model, COPRA-MOS significantly enhanced only Clostridium coccoides-Eubacterium rectale at 24 h with high acetic acid concentration of 87.43 mM. Whereas the chicken simulation model led to low abundance of Enterobacteriaceae but a higher Lactobacillus population with acetic acid concentration of 22.94 mM. These results suggested that COPRA-MOS could potentially apply for poultry industry as it exhibited beneficial effect on cecal fermentation and could be used as a decoy to prevent colonization of a pathogen while to increase the autoaggregation leading to adherence of a probiotic.

Automated summary

This study investigated the production and functions of COPRA-MOS on bacterial aggregation activity and microbial changes in the simulated gastrointestinal tract. The results showed that COPRA-MOS had positive effects on the growth and autoaggregation activity of probiotics, while it had a negative effect on the autoaggregation activity of pathogens. In the simulated gastrointestinal model, COPRA-MOS was beneficial for cecal fermentation, while in the chicken simulation model, it had a positive effect on the population of Lactobacillus.