Journal
CELL STEM CELL
Volume 16, Issue 6, Pages 712-724Publisher
CELL PRESS
DOI: 10.1016/j.stem.2015.04.004
Keywords
-
Categories
Funding
- Leukaemia and Lymphoma Research
- Medical Research Council
- Cancer Research UK
- Biotechnology and Biological Sciences Research Council
- Leukemia Lymphoma Society
- National Institute for Health Research Cambridge Biomedical Research Centre
- Wellcome Trust
- Wellcome Trust-MRC Cambridge Stem Cell Institute
- Canadian Institutes of Health Research
- European Research Council
- University of Cambridge
- Cancer Research UK Institute [C14303/A17197]
- Hutchison Whampoa Limited
- Biotechnology and Biological Sciences Research Council [BB/I00050X/1] Funding Source: researchfish
- Cancer Research UK [12765, 16942] Funding Source: researchfish
- Medical Research Council [G0900951, MC_UU_12021/1, MC_PC_12009, MC_U137761446, MR/M008975/1] Funding Source: researchfish
- National Institute for Health Research [NF-SI-0611-10154] Funding Source: researchfish
- BBSRC [BB/I00050X/1] Funding Source: UKRI
- MRC [MC_UU_12021/1, G0900951, MR/M008975/1, MC_U137761446] Funding Source: UKRI
Ask authors/readers for more resources
Heterogeneity within the self-renewal durability of adult hematopoietic stem cells (HSCs) challenges our understanding of the molecular framework underlying HSC function. Gene expression studies have been hampered by the presence of multiple HSC subtypes and contaminating non-HSCs in bulk HSC populations. To gain deeper insight into the gene expression program of murine HSCs, we combined single-cell functional assays with flow cytometric index sorting and single-cell gene expression assays. Through bioinformatic integration of these datasets, we designed an unbiased sorting strategy that separates non-HSCs away from HSCs, and single-cell transplantation experiments using the enriched population were combined with RNA-seq data to identify key molecules that associate with long-term durable self-renewal, producing a single-cell molecular dataset that is linked to functional stem cell activity. Finally, we demonstrated the broader applicability of this approach for linking key molecules with defined cellular functions in another stem cell system.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available