Journal
MATERIALS TODAY BIO
Volume 16, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.mtbio.2022.100348
Keywords
Artificial periosteum; Quercetin; Osteogenesis; Angiogenesis; Electrospinning
Funding
- National Natural Science Foundation of China [81873988, 82002277, 81672656]
- key Research and Development Program of Hunan Province of China [2021GK2012, 2020GK2008]
- Natural Science Foundation of Changsha City [kq2007069]
- China Postdoctoral Science Foundation [2020M670105ZX]
- Program of the National Natural Science Foundation of Hunan Province [2021JJ40989]
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Artificial periosteum of PLGA/MgO/Quercetin was prepared by electrospinning with highly porous surface and microstructure. The membrane showed excellent biocompatibility and promoted cell proliferation, migration, and osteogenic differentiation. It also facilitated angiogenesis of endothelial progenitor cells. In animal models, optimal osteogenesis and angiogenesis were observed for the PLGA/20 wt% MgO/0.1 wt% Quercetin membrane, indicating its potential for generating artificial periosteum.
Bone nonunion or delayed union, caused by stripping or injuring of periosteum, is the most common sequelae of segmental bone defects. The preservation of periosteum, or the use of periosteal grafts, can significantly improve the integration of bone graft, speeding up the process of bone reconstruction. However, in most cases, periosteum cannot be preserved with bioactivity. Thus, it is pivotal to develop artificial periosteum. In this study, artificial periosteum of PLGA/MgO/Quercetin was prepared by electrospinning. PLGA/MgO/Quercetin membranes were shown to have a highly porous surface and microstructure, as observed by scanning electron microscopy. Along with excellent biocompatibility, PLGA/MgO/Quercetin membranes promoted cell proliferation and migration, as well as osteogenic differentiation of BMSCs (Bone marrow mesenchymal stem cells) in a dose-dependent manner through the activation of Wnt/??-Catenin pathway. The PLGA/MgO/Quercetin membranes, with an appropriate concentration of quercetin (<1 wt%), promoted EPCs (Endothelial progenitor cells) angiogenesis. In a subcutaneous implantation model and rat skull defect model, optimal osteogenesis and angiogenesis function were observed for the PLGA/20 wt% MgO/0.1 wt% Quercetin membrane. In conclusion, PLGA/MgO membranes, with an appropriate concentration of quercetin, show a variety of biological activities and are promising materials for the generation of artificial periosteum.
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