Construction of artificial periosteum with methacrylamide gelatin hydrogel-wharton's jelly based on stem cell recruitment and its application in bone tissue engineering

The presence of periosteum can greatly affect the repair of a bone fracture. An artificial periosteum imitates the biological function of natural periosteum, which is capable of protecting and maintaining bone tissue structure and promoting bone repair. In our artificial periosteum, biocompatible methacrylate gelatin was used to provide the mechanical support of the membrane, E7 peptide added bioactivity, and Wharton's jelly provided the bio-logical activity support of the membrane, resulting in a hydrogel membrane (G-E-W) for the chemotactic recruitment of bone marrow mesenchymal stem cells (BMSCs) and promoting cell proliferation and osteogenic differentiation. In an in vitro experiment, the G-E-W membrane recruited BMSCs and promoted cell proliferation and osteogenic differentiation. After 4 weeks and 8 weeks of implantation in a rat skull defect, the group implanted with a G-E-W membrane was superior to the blank control group and single-component membrane group in both quantity and quality of new bone. The artificial G-E-W membrane recruits BMSC chemotaxis and promotes cell proliferation and osteogenic differentiation, thereby effectively improving the repair efficiency of fractures and bone defects.

Automated summary

The presence of periosteum plays a crucial role in bone fracture repair. An artificial periosteum, composed of biocompatible methacrylate gelatin for mechanical support, E7 peptide for bioactivity, and Wharton's jelly for biological activity support, was developed to recruit bone marrow mesenchymal stem cells (BMSCs) and promote cell proliferation and osteogenic differentiation. In vitro and in vivo experiments demonstrated that the G-E-W membrane effectively recruited BMSCs and promoted bone regeneration. Therefore, the artificial G-E-W membrane shows great potential in enhancing the efficiency of fracture and bone defect repair.