Journal
RMD OPEN
Volume 8, Issue 2, Pages -Publisher
BMJ PUBLISHING GROUP
DOI: 10.1136/rmdopen-2022-002672
Keywords
Sjogren's Syndrome; Polymorphism; Genetic; Autoimmune Diseases; Autoimmunity; Autoantibodies
Categories
Funding
- National Institutes of Health (NIH) [R01AR065953, R01AR074310, P50AR060804, UM1AI144292, P30AR073750, U54GM104938, R01HL113326, P20GM103456, R33AR076803, R21AR079089]
- Presbyterian Health Foundation
- Sjogren's Syndrome Foundation
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The study identified specific transcription profiles in Sjögren's disease (SjD) cases, with downregulation of differentially expressed (DE) transcripts compared to healthy controls. Certain lncRNAs exhibited correlated expression with interferon (IFN)-regulated genes in SjD patients.
ObjectiveSjogren's disease (SjD) is an autoimmune disease characterised by inflammatory destruction of exocrine glands. Patients with autoantibodies to Ro/SSA (SjD(Ro+)) exhibit more severe disease. Long non-coding RNAs (lncRNAs) are a functionally diverse class of non-protein-coding RNAs whose role in autoimmune disease pathology has not been well characterised.MethodsWhole blood RNA-sequencing (RNA-seq) was performed on SjD cases (n=23 Ro/SSA negative (SjD(Ro-)); n=27 Ro/SSA positive (SjD(Ro+)) and healthy controls (HCs; n=27). Bioinformatics and pathway analyses of differentially expressed (DE) transcripts (log(2) fold change >= 2 or <= 0.5; p(adj)<0.05) were used to predict lncRNA function. LINC01871 was characterised by RNA-seq analyses of HSB-2 cells with CRISPR-targeted LINC01871 deletion (LINC01871(-/)(-)) and in vitro stimulation assays.ResultsWhole blood RNA-seq revealed autoantibody-specific transcription profiles and disproportionate downregulation of DE transcripts in SjD cases relative to HCs. Sixteen DE lncRNAs exhibited correlated expression with the interferon (IFN)-regulated gene, RSAD2, in SjD(Ro+) (r >= 0.65 or <=-0.6); four antisense lncRNAs exhibited IFN-regulated expression in immune cell lines. LINC01871 was upregulated in all SjD cases. RNA-seq and pathway analyses of LINC01871(-/)(-) cells implicated roles in cytotoxic function, differentiation and IFN gamma induction. LINC01871 was induced by IFN gamma in a myeloid cell line and regulated by calcineurin/NFAT pathway and T cell receptor (TCR) signalling in primary human T cells.ConclusionLINC01871 influences expression of many immune cell genes and growth factors, is IFN gamma inducible, and regulated by calcineurin signalling and TCR ligand engagement. Altered LINC01871 expression may influence the dysregulated T cell inflammatory pathways implicated in SjD.
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