4.7 Article

Sialyl-Tn Antigen-Imprinted Dual Fluorescent Core-Shell Nanoparticles for Ratiometric Sialyl-Tn Antigen Detection and Dual- Color Labeling of Cancer Cells

Journal

ACS APPLIED NANO MATERIALS
Volume 5, Issue 12, Pages 17592-17605

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acsanm.2c03252

Keywords

cancer; core-shell particles; dual-color labeling; glycan; molecular imprinting

Funding

  1. European Union [721297]
  2. Marie Curie Actions (MSCA) [721297] Funding Source: Marie Curie Actions (MSCA)

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This study presents a strategy for ratiometric STn detection and dual-color cancer cell labeling using molecularly imprinted polymers (MIPs). The synthesized dual fluorescent STn-MIPs allowed for dual-color labeling of cancer cells, providing improved accuracy compared to single-signal MIPs. The concept of the luminescent core-fluorescent MIP shell architecture can be applied to other target analytes.
Sialyl-Tn (STn or sialyl-Thomsen-nouveau) is a carbohydrate antigen expressed by more than 80% of human carcinomas. We here report a strategy for ratiometric STn detection and dual-color cancer cell labeling, particularly, by molecularly imprinted polymers (MIPs). Imprinting was based on spectroscopic studies of a urea-containing green-fluorescent monomer 1 and STn-Thr-Na (sodium salt of Neu5Ac alpha 2-6GalNAc alpha-O-Thr). A few-nanometer-thin green-fluorescent polymer shell, in which STn-Thr-Na was imprinted with 1, other comonomers, and a cross-linker, was synthesized from the surface of red-emissive carbon nanodot (R-CND)-doped silica nanoparticles, resulting in dual fluorescent STn-MIPs. Dual-color labeling of cancer cells was achieved since both red and green emissions were detected in two separate channels of the microscope and an improved accuracy was obtained in comparison with single-signal MIPs. The flow cytometric cell analysis showed that the binding of STn-MIPs was significantly higher (p < 0.001) than that of non-imprinted polymer (NIP) control particles within the same cell line, allowing to distinguish populations. Based on the modularity of the luminescent core- fluorescent MIP shell architecture, the concept can be transferred in a straightforward manner to other target analytes.

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