Detection of silver nanoparticles inside leaf of European beech (Fagus sylvatica L.)

In a greenhouse experiment, silver nanoparticles (Ag-NPs) were applied on European beech (Fagus sylvatica L.) leaves using the droplet application method. Scanning electron microscopy (SEM) analyses showed that after 24 h silver nanoparticles were mostly present in aggregates or as single particles on the surface of the leaf, surrounding or covering the stomata. Analyses of cross sections of the leaf revealed that some silver nanoparticles were adhering to the cell walls of the mesophyll and palisade cells, most likely after penetration into the leaf through the stomata as particles and not as Ag ions. Our preliminary results showed evidence of foliar uptake of silver nanoparticles in European beech. This opens new insights on the ability of trees to take up solid nanosized particles, eventually contained in raindrops, through their leaves, and potentially transport them to other parts of the tree. This study would be helpful for investigating the role of trees in atmospheric ultrafine particle mitigation.

Automated summary

In a greenhouse experiment, silver nanoparticles were applied to European beech leaves and were found to primarily aggregate on the leaf surface, surrounding the stomata. Further analysis revealed that some nanoparticles adhered to the cell walls, suggesting that they were absorbed as particles rather than ions through the stomata. These findings provide new insights into the ability of trees to uptake nanosized particles and their potential transportation within the tree.