Poly-γ-glutamic acid-producing bacteria reduce wheat Cd uptake by promoting Cd transfer from macro-to micro-aggregates in Cd-contaminated soil

Heavy metal immobilization using poly-gamma-glutamic acid-producing bacteria is a novel and environmentally friendly technique for the remediation of heavy metal-contaminated soil. However, only a few studies have investigated the effects of gamma-PGA-producing bacteria on the Cd uptake of wheat plants and the Cd distribution in soil aggregates in Cd-polluted soils. In this study, solution culture and pot experiments were used to investigate the Cd immobilization effect and mechanism of the gamma-PGA-producing bacteria Bacillus subtilis W7 and Bacillus amyloliquefaciens W25. In the two bacteria-inoculated culture media, the concentration of Cd decreased, whereas the pH, cell growth, gamma-PGA production and cell-immobilized Cd significantly increased over time. Strain W25 exhibited a higher ability to produce gamma-PGA and immobilize Cd than strain W7. In the pot experiments, the grain Cd content of wheat was reduced by 24-35% and the DTPA-Cd content was decreased by 22-37% in the rhizosphere soils inoculated with both strains compared to the control. Furthermore, strain W25 had a greater ability to decrease the grain Cd uptake than strain W7. Inoculation with the two strains significantly increased the pH, organic matter content, and urease activity and promoted the migration of Cd from large fractions (> 0.25 mm) to small fractions (< 0.048 mm) and the transformation of available Cd to unavailable Cd in wheat rhizosphere soil. Our results highlight the potential of gamma-PGA-producing bacteria in remediating Cd-polluted soils for safe wheat producing.

Automated summary

Heavy metal immobilization using poly-gamma-glutamic acid-producing bacteria is an environmentally friendly technique for remediating heavy metal-contaminated soil. This study investigated the effects of gamma-PGA-producing bacteria on Cd uptake in wheat plants and Cd distribution in soil aggregates. The results showed that the gamma-PGA-producing bacteria effectively immobilized Cd, with strain W25 exhibiting higher Cd immobilization ability than strain W7. Inoculation with these bacteria also promoted the migration of Cd to smaller fractions and the transformation of available to unavailable Cd in wheat rhizosphere soil.