Journal
JOURNAL OF SEPARATION SCIENCE
Volume 39, Issue 13, Pages 2595-2603Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201600015
Keywords
Automated extraction; Benzodiazepines; Hollow fiber; Liquid chromatography; Microextraction
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Funding
- Tarbiat Modares University
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In this study, two-phase hollow-fiber liquid-phase microextraction and three-phase hollow-fiber liquid-phase microextraction based on two immiscible organic solvents were compared for extraction of oxazepam and Lorazepam. Separations were performed on a liquid chromatography with mass spectrometry instrument. Under optimal conditions, three-phase hollow-fiber liquid-phase microextraction based on two immiscible organic solvents has a better extraction efficiency. In a urine sample, for three-phase hollow fiber liquid-phase microextraction based on two immiscible organic solvents, the calibration curves were found to be linear in the range of 0.6-200 and 0.9-200 mu g L-1 and the limits of detection were 0.2 and 0.3 mu g L-1 for oxazepam and lorazepam, respectively. For two-phase hollow fiber liquid-phase microextraction, the calibration curves were found to be linear in the range of 1-200 and 1.5-200 mu g L-1 and the limits of detection were 0.3 and 0.5 mu g L-1 for oxazepam and lorazepam, respectively. In a urine sample, for three-phase hollow-fiber-based liquid-phase microextraction based on two immiscible organic solvents, relative standard deviations in the range of 4.2-4.5% and preconcentration factors in the range of 70-180 were obtained for oxazepam and lorazepam, respectively. Also for the two-phase hollow-fiber liquid-phase microextraction, preconcentration factors in the range of 101-257 were obtained for oxazepam and lorazepam, respectively.
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