Journal
JOURNAL OF SEPARATION SCIENCE
Volume 39, Issue 21, Pages 4158-4165Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201600679
Keywords
Ginkgo biloba; Hydroxyl radical scavengers; Molecular devices
Categories
Funding
- National Natural Science Foundation of China [31500279, 31400453, 81303280, 31370374, 81373899]
- Natural Science Foundation of Jilin Province [20130413043GH, 20130521013ZH, 20150520140JH, 20160101209JC]
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Hydroxyl radicals are the most reactive free radical of human body, a strong contributor to tissue damage. In this study, liquid chromatography coupled to electrospray ionizationmass spectrometry was applied to screen and identify hydroxyl radical scavengers from the total flavonoids of Ginkgo biloba leaves, and high-performance counter current chromatography was used to separate and isolate the active compounds. Furthermore, molecular devices were used to determine hydroxyl radical scavenging activities of the obtained hydroxyl radical scavengers and other flavonoids from G. biloba leaves. As a result, six compounds were screened as hydroxyl radical scavengers, but only three flavonoids, namely, rutin, cosmos glycosides and apigenin-7-O-Glu-4'-O-Rha, were isolated successfully from total flavonoids by high-performance counter current chromatography. The purities of the three obtained compounds were over 90%, respectively, as determined by liquid chromatography. Molecular devices with 96-well microplates evaluation indicated that the 50% scavenging concentration values of screened compounds were lower than that of other flavonoids, they performed greater hydroxyl radical scavenging activity, and the evaluation effects were consistent with the liquid chromatography with mass spectrometry screening results. Therefore, chromatography combined with molecular devices is a feasible and an efficient method for systematic screening, identification, isolation, and evaluation of bioactive components in mixture of botanical medicines.
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