Journal
JOURNAL OF SEPARATION SCIENCE
Volume 39, Issue 19, Pages 3736-3744Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201600535
Keywords
Liquid chromatography; Open tubular columns; Porous layers; Zwitterionic polymers
Categories
Funding
- National Key Scientific Instrument and Equipment Development Project [2012YQ120044]
- State Key Program of National Natural Science of China [21235005]
- National Natural Science Foundation of China [21475044]
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A 3 m zwitterionic polymeric porous layer open tubular column (3 m x 25 mu m id x 375 mu m od) with a polymeric porous layer thickness of 4 mu m was fabricated by the copolymerization of [2-(methacryloyloxy) ethyl] dimethyl-(3-sulfopropyl) ammonium hydroxide and N,N'-methylenebis(acrylamide). The effects of the diameter of the capillary, reaction temperature, and polymerization time on the preparation of the open tubular column were investigated. Characterized by scanning electron microscopy, the zwitterionic layer was observed to be rough and throughout the fused-silica capillary homogenously, which increased the phase ratio. The separation of neutral, basic, and acidic compounds demonstrates the strong hydrophilicity of the poly[2-(methacryloyloxy) ethyl] dimethyl-(3-sulfopropyl) ammonium hydroxide coating. In addition, the poly[2-(methacryloyloxy) ethyl] dimethyl-(3-sulfopropyl) ammonium hydroxide porous layer open tubular column was applied for the analysis of flavonoids from the rootstalk of licorice, revealing the potential in separating complex samples. The relative standard deviation of retention time for run-to-run (n = 5), day-to-day (n = 3), and column-to-column (n = 3) of toluene, N, N-dimethylformamide, formamide, and thiourea were below 1.2%, exhibiting good repeatability.
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