Journal
JOURNAL OF SEPARATION SCIENCE
Volume 39, Issue 15, Pages 2963-2969Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201600345
Keywords
Derivatization; Formaldehyde; Liquid-liquid extraction; Liver tissue
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A simple high-performance liquid chromatography method has been developed for the determination of formaldehyde in human tissue. FA Formaldehyde was derivatized with 2,4-dinitrophenylhydrazine. It was extracted from human tissue with ethyl acetate by liquid-liquid extraction and analyzed by high-performance liquid chromatography. The calibration curve was linear in the concentration range of 5.0-200 mu g/mL. Intra-and interday precision values for formaldehyde in tissue were <6.9%, and accuracy (relative error) was better than 6.5%. The extraction recoveries of formaldehyde from human tissue were between 88 and 98%. The limits of detection and quantification of formaldehyde were 1.5 and 5.0 mu g/mL, respectively. Also, this assay was applied to liver samples taken from a biopsy material.
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